福建农业学报2024，Vol.39Issue(10)：1152-1161,10.DOI:10.19303/j.issn.1008-0384.2024.10.006
一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定
Isolation and Identification of a Goose-origin Muscovy Duck Reovirus Causing White Pinhead Necrotic Foci on Liver and Spleen in Geese
摘要
Abstract
[Objective]Pathogen of the disease characterized by white pinhead necrotic foci on the liver and spleen in geese was isolated and identified.[Methods]PCR was used to screen possible pathogens from tissue specimens of diseased birds.Subsequently,the suspect pathogens were inoculated in Muscovy duck embryos and Muscovy duck embryo fibroblasts(MDEF)cells to identify positive isolate for further confirmation by RT-PCR,genes sequencing,and a challenge test on gooselings.[Results]A strain of goose-origin Muscovy duck reovirus(Go-MDRV),JS2022,was obtained and inoculated into Muscovy duck embryos causing stunted growth.After 6 viral passages,mortality of the infected embryos stabilized to occur in 3-5 d post-inoculation.Haemorrhagic spots and the typical pinhead necrotic foci were visible on the liver of the dead embryos.On MDEF cells,the inoculation induced cytopathic cell shrinkage and disintegration.By comparing the nucleotides of σB and σC of JS2022 with those of Go-MDRV,Muscovy duck reovirus(MDRV),novel duck reovirus(NDRV),and avian reovirus(ARV),the greatest homology as found between JS2022 and Go-MDRV with the ranges of 99.1%-99.5%on σB and 99.3%-99.9%on σC.In the challenge test,the infected gooselings had symptoms identical to those shown in the clinical cases.[Conclusion]A strain of Go-MDRV isolated from the diseased geese bearing the specific symptom of white necrotic foci on the liver and spleen was identified and code-named JS2022.关键词
鹅源番鸭呼肠孤病毒/分离/鉴定/σB基因/σC基因/同源性比较Key words
Go-MDRV/isolation/identification/σBgene/σCgene/homology comparison分类
农业科技引用本文复制引用
郑欣,曾显成,陈少莺,陈仕龙,胥焯然,宫晓,江丹丹,程晓霞,朱小丽,郑敏,曾丽,肖世峰..一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定[J].福建农业学报,2024,39(10):1152-1161,10.基金项目
福建省农业科学院科技创新团队建设项目(CXTD2021034) （CXTD2021034）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021018、XTCXGC2021012) （XTCXGC2021018、XTCXGC2021012）
福建省农业科学院对外合作项目(DWHZ2024-05) （DWHZ2024-05）
福建省科技计划公益类专项(2021R1026009) （2021R1026009）
