福建农业学报2024，Vol.39Issue(10)：1179-1188,10.DOI:10.19303/j.issn.1008-0384.2024.10.009
茶树应答茶小绿叶蝉为害的蛋白质组分析
Proteomics of Tea Plant in Response to Infestation by Empoasca vitis
摘要
Abstract
[Objective]Proteins related to the response of Duyun Maojian native tea plant infested by Empoasca vitis were studied.[Method]Leaves of Duyun Maojian native tea plant were artificially infested by E.vitis Göthe for 0,12,24,36,and 48 h.Tandem mass tag combined with liquid chromatography-tandem mass spectrometry was applied to detect the proteins generated in the leaves by the infestation.[Results]The chemical analysis identified 2 893 proteins that included 0,1,848,and 849 differential expression proteins(DEPs)in the leaf specimens sampled at the time periods that lasted from 0 h to 12 h,24 h,36 h,and 48 h,respectively.Of the 2 622 annotated proteins,2 360 were explained to the GO database and 1 232 to the KEGG database.The GO and KEGG analyses indicated that the tea plants might be resistant to E.vitis infestation through the enzymes,such as CMK,FPPS,GPPS,GGPPS,peroxidase,pectinesterase,and allene-oxide cyclase,the biosynthesis of sesquiterpenoid,triterpeoid,monoterpenoid,and diterpenoid,and/or the biosynthesis pathways of sesquiterpenoid,triterpenoid,terpenoid backbone,and monoterpenoid.[Conclusion]Four DEPs,i.e.,CMK,FPPS,GPPS,and GGPPS,and terpenoids might play important roles in the response and defense of the tea plant against E.vitis Göthe.关键词
茶树/茶小绿叶蝉/蛋白质组/TMT/差异蛋白Key words
tea plant/Empoasca vitis Gothe/proteomics/tandem mass tag/differential expression protein分类
农业科技引用本文复制引用
王芬,喻佩,翟媛,李传刚,罗思妤,裴会敏..茶树应答茶小绿叶蝉为害的蛋白质组分析[J].福建农业学报,2024,39(10):1179-1188,10.基金项目
国家自然科学基金项目(32260080) （32260080）
贵州省教育厅项目(QNYSKYTD2018006) （QNYSKYTD2018006）
黔南民族师范学院项目(2019xjg0303、2018xjg0520、QNSY2018ZJ006、QNYSXXK2018005) （2019xjg0303、2018xjg0520、QNSY2018ZJ006、QNYSXXK2018005）
