福建农业学报2024，Vol.39Issue(11)：1213-1217,5.DOI:10.19303/j.issn.1008-0384.2024.11.001
水稻粒长基因GS3的鉴定及利用分析
Identification and Sequencing of Gene Related to Length of Rice Grain
摘要
Abstract
[Objective]The gene that regulates the length of a rice grain,which directly relates to crop yield,was identified and sequenced for breed improvement.[Method]Through map-based cloning,499 recombinant inbred lines derived from the interspecific cross between Jiafuzhan(indica)and Nipponokin(japonica)were employed to identify the quantitative trait loci associated with the phenotypic trait.[Result]A major plasmid length of GS3 was 1851 kb,detected in the recombinant inbred line population located approximately within the physical distance 16310-18161 kb.Since only the 48th base of the second exon was mutated from C to A between the genes of Jiafuzhan and Nipponbare,protein translation was no longer necessary.Further analysis on Jiazao No.1 and Jiafuxiangzhan bred using the GS3 showed no significant difference than the wild type Jiafuzhan in grain length,width,or length-width ratio.[Conclusion]The long-grain gene GS3 could be detected in the recombinant inbred lines of Jiafuzhan×Nipponbare,and could be stably inherited and applied.The information on GS3 obtained in this study was of value for future studies to genetically improve rice production.关键词
水稻/长粒/GS3/基因鉴定Key words
rice/grain length/GS3/gene mapping分类
农业科技引用本文复制引用
郑长林,官华忠,赵锦祥,毛大梅,陈志伟,杨德卫..水稻粒长基因GS3的鉴定及利用分析[J].福建农业学报,2024,39(11):1213-1217,5.基金项目
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021019) （XTCXGC2021019）
福建省农业科学院东西部合作项目(DKBF-2024-12) （DKBF-2024-12）
