福建农业学报2024，Vol.39Issue(11)：1228-1235,8.DOI:10.19303/j.issn.1008-0384.2024.11.003
克氏原螯虾prx 6基因抗鲶爱德华氏菌侵染的分子机制研究
Antimicrobial Function of Crayfish prx 6 on Edwardsiella ictaluri
摘要
Abstract
[Objective]Function of peroxiredoxin 6 gene(prx 6)in the antimicrobial innate immune response mechanism of Procambarus clarkii against Edwardsiella ictaluri was studied.[Methods]To confirm the involvement of prx 6 in the immunological response,gene expressions in the haemocytes,hepatopancreas,gills,and intestines of the P.clarkii were examined.Double-strand RNA(dsRNA)of the antimicrobial peptide(Amp)gene in P.clarkii,prx 6,was prepared by in vitro transcription in crayfish blood sinusoids using sterile syringes with the effect verified by qRT-PCR analysis.In a challenge test,survival rate of the E.ictaluri-inoculated crayfish was determined by a RNAi assay with the changes in prx 6 expression analyzed by qRT-PCR.[Results]The upregulated expressions of prx 6 in various crayfish tissues after the E.ictaluri stimulation indicated a positive association of the gene with the pathogenic invasion.Furthermore,an injection of the in vitro prepared dsRNA significantly suppressed the expression of prx 6 in the hepatopancreas confirming the response of the crayfish.The RNAi assay also showed a significant lower survival rate of the crayfish in the E.ictalurid-inoculation group than that in the dsGFP control indicating a significant effect of prx 6 in combating the bacterial infections.Additionally,in the challenge test some other endogenous Amp genes,such as ALF 4,ALF 1,crustin 1,crustin 2,lys,and lectin 2,were significantly downregulated in the E.ictaluri-inoculated crayfish in comparison with the dsGFP-inoculated counterparts.It was speculated that prx 6 contributed to the crayfish resistance to the infection by E.ictaluri through modulating Amp genes resulting in an improved survival.It was hypothesized that prx 6 participated in the innate immune response of crayfish against E.ictaluri through regulations on the endogenous Amp genes.关键词
克氏原螯虾/RNA干扰/先天免疫/prx 6基因/抗菌肽基因/鲶爱德华氏菌Key words
Procambarus clarkii/RNAi/innate immunity/prx 6/Amps/E.ictaluri分类
农业科技引用本文复制引用
秦仕宇,金博阳,李倩倩,张明达,文静,沈秀丽,杜志强..克氏原螯虾prx 6基因抗鲶爱德华氏菌侵染的分子机制研究[J].福建农业学报,2024,39(11):1228-1235,8.基金项目
内蒙古自治区自然科学基金项目(2024MS03051) （2024MS03051）
国家自然科学基金项目(32060834) （32060834）
内蒙古科技大学2022年基本科研业务费专项资金(028) （028）
