福建农业学报2024，Vol.39Issue(11)：1265-1279,15.DOI:10.19303/j.issn.1008-0384.2024.11.008
转录组和代谢组联合分析茶树对茶饼病病原菌的防御反应
Responses of Tea Plants to Blister Blight Analyzed Using Transcriptome and Metabolome
摘要
Abstract
[Objective]Resistance mechanism and associated genes of tea plants in response to blister blight infection were studied based on transcriptome as well as metabolome.[Methods]Differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in leaves of healthy(CK)and blister blight-infected(TB)tea plants were compared by using both transcriptome and metabolome.[Results]Between CK and TB,1 009 DEGs associated with the cell wall metabolism and the regulations of chitinase,oxidoreductase,and xyloglucan:xyloglucanosyltransferase activities were identified by a GO enrichment analysis.The KEGG analysis showed the DEGs significantly enriched in the pathways of flavonoid biosynthesis,phenylpropanoid biosynthesis,amino sugar and nucleotide sugar metabolism,glycerolipid metabolism,and stilbenoid,diarylheptanoid,and gingerol biosynthesis.Forty-seven transcription factors in the DEGs,which belonged to 21 transcription factor families including bHLH,SBP,AP2/ERF-AP2,and MYB,might be the genes significantly involved in the resistance of a tea plant to blister blight.The 353 DAMs identified by using the widely targeted metabolomics were enriched mainly in the flavonoid biosynthesis and lysine biosynthesis as well as the alanine,aspartate,and glutamate metabolism pathways.The transcriptome and metabolome revealed significant co-enrichments in the flavonoid biosynthesis,phenylpropanoid biosynthesis,and stilbenoid,diarylheptanoid,and gingerol biosynthesis pathways.Among the selected 20 DEGs and 15 DAMs associated with the phenylpropanoids and flavonoids biosynthesis pathways,DEGs such as CSS0011741(4CL),CSS0002940(DFR),CSS0015968(DFR),and CSS0010687(ANS)were upregulated,while DAMs such as phloretin,phlorizin,4-Hydroxystyrene,p-Coumaroyl quinic acid,dihydromyricetin,epigallocatechin,and peonidin 3-O-glucoside were accumulated in the infected leaves.[Conclusion]The DEGs in the phenylpropanoid and flavonoid biosynthesis pathways might play a crucial role in the response of tea plants to blister blight.And the DAMs,such as phloretin,phlorizin,and epigallocatechin,might be the secondary metabolites in tea plants that participated closely in the resistance mechanism.关键词
茶饼病/转录组/代谢组/差异表达基因/差异代谢物Key words
Exobasidium vexans/transcriptome/metabolome/differentially expressed genes/differentially accumulated metabolites分类
农业科技引用本文复制引用
姚玉仙,张明泽,舒烨,罗福丹,全昌彬,刘荣,向志攀,梅鑫..转录组和代谢组联合分析茶树对茶饼病病原菌的防御反应[J].福建农业学报,2024,39(11):1265-1279,15.基金项目
贵州省基础研究(自然科学)项目(黔科合基础[2020]1Y120) （自然科学）
贵州省教育厅自然科学基金项目(黔教合KY字[2019]211) （黔教合KY字[2019]211）
贵州省教育厅贵州省高等学校茶树特征性成分研究重点实验室项目(黔教技[2023]027号) （黔教技[2023]027号）
贵州省大学生创新创业训练计划项目(S202210670021) （S202210670021）
