福建农业学报2024，Vol.39Issue(11)：1280-1288,9.DOI:10.19303/j.issn.1008-0384.2024.11.009
福建地区猕猴桃细菌性溃疡病菌的生物学特征及其杀菌剂筛选
Biological Characteristics and Effective Bactericides of Pseudomonas syringae pv.actinidiae
摘要
Abstract
[Objective]Pseudomonas syringae pv.actinidiae(Psa),pathogen of the bacterial canker disease severely affecting the kiwifruit production in Fujian,was studied and effective bactericidal agents selected.[Methods]Colony count on petri dishes and pathogenicity by leaf stabbing inoculation were used to determine the conditions optimal for Psa propagation.Control efficacy of 9 bactericides at the supplier's recommended dose was observed by spraying the agents separately on the Psa-inoculated kiwifruit leaves.[Results]The Psa population increased in culture at 25℃ at a relatively constant rate for 48-72 h but did not survive under 70℃ for 10 min.Either LBA or NA medium could be used appropriately for the culture,and LBA for the inoculation.The strain dependent pathogenicity of different strains of Psa,FJ02,FJ04,and FJ08,on kiwifruit and tomato leaves were substantial.Of the 9 bactericides tested,5 were more potent:(a)200×aqueous suspension of 6 billion Bacillus amyloliquefaciens gemmae·mL-1+1 000×aqueous dilution of 3%ZhongShengmycin,(b)1 000×solution of 52%methionate·ZhongShengmycin wettable powder,(c)500×solution of 10%kasugamycin wettable powder,(d)1 000×aqueous dilution of 3%ZhongShengmycin,and(e)500×solution of 18%copper rosinate emulsion.Respectively,they provided the prevention effects of 82.99%,79.45%,64.92%,58.39%,and 43.97%and the control effects of 76.33%,65.08%,85.80%,52.65%,and 74.85%.The germicidal treatments were deemed safe on kiwifruit leaves.[Conclusion]The biological characteristics Psa and the effective canker preventive and control bactericides for kiwifruits were successfully determined.The tested agents(a),(b),and(c)were recommended for field application.关键词
猕猴桃细菌性溃疡病/猕猴桃细菌性溃疡病菌/生物学特征/致病力/杀菌剂Key words
Kiwifruit bacterial canker/Pseudomonas syringae pv.actinidiae/biological characteristics/pathogenicity/bactericides分类
农业科技引用本文复制引用
甘林,兰成忠,占仕发,陈艳辉,代玉立,杨秀娟..福建地区猕猴桃细菌性溃疡病菌的生物学特征及其杀菌剂筛选[J].福建农业学报,2024,39(11):1280-1288,9.基金项目
国家重点研发计划项目(2021YFC2600402) （2021YFC2600402）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021011) （XTCXGC2021011）
福建省农业科学院科技创新平台专项(CXPT202105) （CXPT202105）
