福建农业学报2024，Vol.39Issue(11)：1289-1297,9.DOI:10.19303/j.issn.1008-0384.2024.11.010
桑葚果腐病病原菌的分离鉴定及抑菌植物提取物筛选
Identification and Natural Antibacterial Agent of Mulberry Rot Pathogen
摘要
Abstract
[Objective]Pathogen of the fruit rot on mulberries was isolated and identified,and effective antibacterial plant extracts for the disease prevention and control selected.[Method]Tissue separation method was applied to isolate potential pathogens from the infected mulberries.The morphology and molecular biology of the isolates were examined for pathogen identification.Antibacterial activity of 54 plant ethanol extracts was tested to compare the inhibition on the growth of the isolate mycelia.Virulence of the most potent extracts at gradient concentrations was determined along with microscopic observations.[Result]Growth of the identified pathogen,Mucor fragilis was significantly inhibited at a rate of 77.72%by the extract of Artemisia caruifolia on a dosage of 1 mg·mL-1 rendering an EC50 at 0.063 mg·mL-1,and also at a rate of 49.06%by Salvia uliginosa with an EC50 at 0.107 mg·mL-1.In addition to a higher inhibition rate,the A.caruifolia extract induced significant mycelial distortions on the pathogens as shown under the microscope.[Conclusion]Significant antifungal activities against the mulberry rot-causing M.fragilis were found of the ethanol extracts of A.caruifolia and S.uliginosa.With a toxicity of EC50 at 0.063 mg·mL-1 and significant deformation on the mycelia of the pathogen,A.caruifolia was considered the choice candidate as a biocontrol agent against the disease.关键词
桑葚果腐病/植物提取物/抑菌活性/毒力Key words
Mulberry rot disease/plant extracts/antibacterial activity/toxicity分类
农业科技引用本文复制引用
李青,宋春茶,罗建梅,杨态娇,聂闪闪,张东华,刘丽,胡世俊,闫晓慧..桑葚果腐病病原菌的分离鉴定及抑菌植物提取物筛选[J].福建农业学报,2024,39(11):1289-1297,9.基金项目
国家自然科学基金(32160378) （32160378）
云南省高层次人才项目(YN-WR-QNBJ-2018-300) （YN-WR-QNBJ-2018-300）
西南林业大学博士科研启动经费项目(111711、111712) （111711、111712）
