福建中医药2025，Vol.56Issue(1)：11-16,6.DOI:10.13260/j.cnki.jfjtcm.2025.01004
理气通便方对气滞型功能性便秘患者的疗效及肠道菌群的影响
Effect of Liqi Tongbian Decoction on the Efficacy and Intestinal Flora of Patients with Qi-Stagnation Type Functional Constipation
摘要
Abstract
Objective:To explore the effect of Liqi Tongbian Decoction on functional constipation(FC)patients with Qi-stagna-tion type functional constipation(FC)and its effect on intestinal flora.Methods:A total of 34 outpatients and inpatients with FC of Qi-stagnation type diagnosed in the Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine from January to December 2021 were selected as the treatment group.The response rate of complete spontaneous bowel movements(CS-BMs),efficacy of traditional Chinese medicine(TCM)syndromes,the Patients'Assessment of Quality of Life in Constipation(PAC-QOL)score,and the occurrence of adverse reactions were assessed after 4 weeks of treatment with Liqi Tongbian Decoction,and 20 healthy individuals were recruited as the normal group.16S rDNA sequencing technology was used to analyze the Alpha polymor-phism of the intestinal flora of the treatment group before and after treatment and the normal group to determine the Chao1 index and Simpson index,and to determine the relative abundance of the intestinal flora for the analysis of the species composition,variability of butyric acid-producing bacterial genera,characteristic microbiota,and the analysis of the prediction of the gene function of the intes-tinal flora.Results:The total CSBMs response rate of the treatment group was 61.76%,the total effective rate of TCM syndrome was 88.24%,and the PAC-QOL score of the treatment group improved after treatment(P<0.05),and there were no adverse reactions.The top 3 species in terms of relative abundance of intestinal flora at the phylum level in the treatment group before and after treatment and in the normal group were Firmicutes,Bacteroidetes and Proteobacteria,and the top 3 species at the genus level were Bacteroide-tes,Faecalibacterium and Escherichia Shigella.Compared with the normal group,the pre-treatment Chao1 index and Simpson's index of the treatment group increased(P<0.05),and the relative abundance level of butyric acid-producing bacterial genera decreased(P<0.05),with Prevotellaceae,Dialistella,Oscillospiraceae,Lactobacillaceae,Lactobacillus,Veillonellaceae,etc.as characteristic bacte-ria.The function of intestinal flora reduced by sucrose degradation Ⅲ(PWY-621),pyruvate fermentation into acetic acid and lactic ac-id(PWY-5100)(P<0.05).Compared with the pre-treatment period,the differences in Chao1 index and Simpson's index were not sta-tistically significant in the treatment group(P<0.05),while the relative abundance level of butyric acid-producing bacterial genera in-creased(P<0.05),and the characteristic bacteria were Clostridia,Lachnospiraceae,Lachnospirales,Firmicutes and Blautia.The func-tional levels of sucrose degradation Ⅲ(PWY-621),pyruvate fermentation into acetic acid and lactic acid(PWY-5100)increased(P<0.05).Conclusion:Intestinal flora disturbance may be an important pathogenesis of Qi-stagnation type of functional constipation,and Brautella and butyric acid-producing bacterial genera may be the key targets of Liqi Tongbian Decoction.关键词
功能性便秘/理气通便方/肠道菌群/16S rDNAKey words
Liqi Tongbian Decoction/functional constipation/intestinal flora/16S rDNA引用本文复制引用
胡露楠,刘启鸿,黄雅滢,许燕城,方文怡,赵培琳,柯晓..理气通便方对气滞型功能性便秘患者的疗效及肠道菌群的影响[J].福建中医药,2025,56(1):11-16,6.基金项目
国家自然科学基金面上项目(82274282) （82274282）
中央引导地方科技发展资金项目(2023L3013) （2023L3013）
福建省名中医柯晓工作室建设项目(闽卫中医[2022]112号) （闽卫中医[2022]112号）
