辐射研究与辐射工艺学报2025，Vol.43Issue(1)：94-102,9.DOI:10.11889/j.1000-3436.2024-0049
60Co-γ射线辐照灭菌对连翘质量影响的多元评价
Multiple evaluation of the effect of 60Co-γ irradiation sterilization on the quality of Fructus forsythiae
摘要
Abstract
The aim of this study was to investigate the effect of 60Co-γ irradiation sterilization on the quality of Fructus forsythiae.The medicinal powder of Fructus forsythiae was used at different doses.Ingredient concentration,high performance liquid chromatography(HPLC)finger print and antibacterial activity of water extract against Staphylococcus aureus were used before and after irradiation for comparison.A near-infrared spectrum(NIR)consistency model of Fructus forsythiae was established by near-infrared spectroscopic methodology and the optimum dose of 60Co-γ irradiation was investigated.The results showed that there was very significant difference(p<0.01)in the contents of the two compounds(forsythiaside A and forsythiaside I)compared to unirradiated samples.The similarity of HPLC fingerprints was decreased.The water extract's effectiveness against Staphylococcus aureus before and after irradiation in each sample showed no significant difference(p>0.05).The NIR consistency evaluation model was established to determine the irradiated samples.The microbial populations could be reduced to a lower degree at 5 kGy absorbed dose irradiation.60Co-γ irradiation sterilization would affect the quality of Fructus forsythiae at 10 kGy dose.It is recommended that the irradiation sterilization dose not exceed 5 kGy.关键词
连翘/60Co-γ射线/辐照灭菌/指纹图谱/抗菌活性/近红外光谱Key words
Fructus forsythiae/60Co-γ ray/Radiation sterilization/Finger print/Antibacterial activity/Near infrared spectrum分类
能源科技引用本文复制引用
毛腾霄,伍欣然,张聪聪,程龙,郑娇娇,代琪,何江..60Co-γ射线辐照灭菌对连翘质量影响的多元评价[J].辐射研究与辐射工艺学报,2025,43(1):94-102,9.基金项目
四川省药品监督管理局科技计划项目(2022012)资助 Supported by Drug Science and Technology Plan Project of Sichuan Provincial Drug Administration(2022012) （2022012）
