国际医药卫生导报2025，Vol.31Issue(3)：前插2,354-359,7.DOI:10.3760/cma.j.cn441417-20240625-03001
miR-155/Shh/Gli1信号通路在扩张型心肌病CD4+T细胞介导免疫应答中的作用机制
Mechanism of miR-155/Shh/Gli1 signaling pathway in the immune response mediated by CD4+T cells in dilated cardiomyopathy
摘要
Abstract
Objective To explore the mechanism of the miR-155/Shh/Gli1 signaling pathway in CD4+T cells in the pathogenesis and development of dilated cardiomyopathy(DCM).Methods This study was a clinical cohort study.Fifty patients with DCM treated in the Second Hospital of Tianjin Medical University from January 2018 to December 2023 were selected as a DCM group,and 5 0 healthy subjects of the same age during the same period were selected as a healthy control group(HD group).In the DCM group,therewere26malesand24females,aged(49.7±4.9)years.In the HD group,there were 25 males and 25 females,aged(50.8±4.3)years.The expression of CD4+T cells in DCM patients was analyzed,and next generation sequencing(NGS)was performed on CD4+T cells of the peripheral blood from different subgroups to verify the expressions of signaling pathway proteins.The role of miR-155 in DCM was investigated by interfering with its expression level in CD4+T cells.Independent sample t test and Pearson correlation analysis were used for statistical analysis.Results CD4+T cells were actively expressed in DCM patients[(45.5±5.9)％vs.(37.1±6.3)％](t=6.862,P＜0.001),and the expression of miR-155 in CD4+T cells was elevated(P＜0.05).The increased expression of miR-155 promoted the elevation of endoplasmic reticulum stress(ERS)and the expressions of proteins Shh and Gli1 in the Hedgehog signaling pathway.Treatment with Shhagonists further promoted the ERS response.Conclusion The miR-155/Shh/Gli1 signaling pathway may be involved in the abnormal expression of CD4+T cells in DCM.关键词
扩张型心肌病/CD4+T细胞/miR-155/内质网应激Key words
Dilated cardiomyopathy/CD4+T cells/miR-155/Endoplasmic reticulum stress引用本文复制引用
董福强,滕广帅,霍宁,刘长乐..miR-155/Shh/Gli1信号通路在扩张型心肌病CD4+T细胞介导免疫应答中的作用机制[J].国际医药卫生导报,2025,31(3):前插2,354-359,7.基金项目
天津市自然科学基金(21JCYBJC01460) （21JCYBJC01460）
天津市教委科研计划(自然科学)(2023KJ028) （自然科学）
天津市医学重点学科(专科)建设项目(TJYXZDXK-029A) （专科）
天津医科大学第二医院重点实验室项目(2019ZDSYS09) Natural Science Foundation of Tianjin Science and Technology Commission(21JCYBJC01460) （2019ZDSYS09）
Tianjin Education Commission Scientific Research Program(Natural Science)(2023KJ028) （Natural Science）
Tianjin Medical Key Discipline(Specialty)Construction Project(TJYXZDXK-029A) （Specialty）
Key Laboratory Project of the Second Hospital of Tianjin Medical University(2019ZDSYS09) （2019ZDSYS09）
