西部中医药2025，Vol.38Issue(1)：1-6,6.DOI:10.12174/j.issn.2096-9600.2025.01.01
蝉芩颗粒对感染后咳嗽豚鼠神经源性炎症的影响
Influence of Chanqin Granules on Neurogenic Inflammation in Guinea Pigs with Post Infectious Cough
摘要
Abstract
Objective:To discuss the effects of Chanqin granules on neurogenic inflammation in guinea pigs with post infectious cough(PIC)mediated by iNOS/NO-cGMP-PKG signaling pathway.Methods:After establishing the models of H1N1 virus infection by nasal dripping,the guinea pigs were randomized into the blank group,the model group,high,moderate and low doses groups of Chanqin granules,and Asmeton group with six in each group.The blank group and the model group were drenched with physiological saline;high,moderate and low doses groups of Chanqin granules accepted intragastric administration of the medicine at the dose of 15.20,7.60 and 3.80 g/(kg·d)respectively;and Asmeton group was given compound methoxyphenamine hydrochloride capsules at the dose of 25.20 mg/(kg·d)by gavage,they accepted 14 consecutive days of intervention.By the end of intervention,ELISA was adopted to detect the expressions of SP,IL-1β,IL-6,TNF-α and ICAM-1 in the serum;qPCR method was applied to measure the levels of iNOS,sGC and PKG mRNA in dorsal root ganglion of guinea pigs;the expressions of TRPV1 protein in dorsal root ganglion of guinea pigs were tested using WB and IHC.Results:Compared with the blank group,the expressions of SP,IL-6,IL-1β,TNF-α and ICAM-1 in the serum were raised,and the levels of iNOS,sGC and PKG mRNA,as well as TRPV1 protein in the model group(P<0.01);compared with the model group,the expressions of SP,IL-6,IL-1β,TNF-α and ICAM-1 in the serum of high dose group of Chanqin granules and Asmeton group were lowered,as well as the levels of iNOS,sGC and PKG mRNA,and TRPV1 protein(P<0.05),the expressions of SP,IL-6,TNF-α and ICAM-1 in the serum and the levels of iNOS mRNA and TRPV1 protein in dorsal root ganglion of guinea pigs were lowered in the moderate dose group of Chanqin granules(P<0.05),the expressions of SP and IL-6 in the serum and the levels of TRPV1 protein in dorsal root ganglion were reduced(P<0.05);Compared with Asmeton group,the expressions of IL-6 were raised(P<0.05)while the expressions of TRPV1 protein in dorsal root ganglion were lowered in high dose group of Chanqin granules(P<0.05),the levels of IL-6 and IL-1β,the expressions of iNOS,sGC and PKG mRNA were increased in moderate and low doses groups of Chanqin granules(P<0.05),the levels of TNF-α and ICAM-1 were elevated in low dose group of Chanqin granules(P<0.05).Conclusion:Chanqin granules could inhibit TRPV1 pathway and control the release of SP by regulating iNOS/NO-cGMP-PKG signaling pathway,so as to relieve neurogenic inflammation in PIC guinea pigs.关键词
感染后咳嗽/神经源性炎症/蝉芩颗粒/iNOS/NO-cGMP-PKG信号通路/豚鼠/瞬时受体电位香草酸亚型1Key words
post infectious cough/neurogenic inflammation/Chanqin granules/iNOS/NO-cGMP-PKG signaling pathway/guinea pigs/TRPV1分类
医药卫生引用本文复制引用
石炜弘,窦丹波,葛文,吕俊,余小萍,沈若冰..蝉芩颗粒对感染后咳嗽豚鼠神经源性炎症的影响[J].西部中医药,2025,38(1):1-6,6.基金项目
国家自然科学基金(81904127) （81904127）
浦东新区"国家中医药发展综合改革试验区"建设项目(PDZY-2019-0203). （PDZY-2019-0203）
