西部中医药2025，Vol.38Issue(1)：73-79,7.DOI:10.12174/j.issn.2096-9600.2025.01.15
基于网络药理学探讨四妙勇安汤治疗糖尿病足的作用机制
Mechanism of Simiaoyong'an Decoction in the Treatment of Diabetic Foot Based on Network Pharmacology
摘要
Abstract
Objective:To explore the mechanism of Simiaoyong'an decoction in the treatment of diabetic foot based on network pharmacology.Methods:TCMSP was utilized to screen the active ingredients of Jinyinhua(Lonicerae japonicae flos),Xuanshen(Scrophulariae radix),Danggui(Angelicae sinensis radix)and Gancao(Glycyrrhizae radix et rhizoma);PubChem and SwissTargetPrediction database were applied in the screen of active ingredients of the drugs;disease-related targets were obtained from GeneCards and OMIM,and by intersecting the drug targets with the disease targets,the potential targets for drugs action on the disease were identified,introduced into STRING database to construct protein-protein interactions(PPI)network,R4.1.0 software was used to extract the network core genes,and perform GO and KEGG pathway enrichment analysis of the potential targets;KEGG network diagram was built using Cytoscape 3.7.2 software.Results:The study has yielded 126 active ingredients from the decoction,690 related targets and 175 potential targets for the drug acting on the disease,the core targets contain ALB,Akt1,MAPK3,VEGFA,EGFR,TNF and CASP3;the core targets are involved in positive regulation of negative ion transport,peptidylserine modification,peptidylserine phosphoryla-tion and other biological processes,and the sites of action mainly cover membrane rafts,membrane microdomains,vesicles,secretory granules and apical cells,the molecular functions include protein serine/threonine kinase activity,protein tyrosine kinase activity,and the main pathways contain MAPK,PI3K/Akt,AGE-RAGE,Ras and HIF-1.Conclusion:The medicine could treat diabetic foot via multi-component,multi-target and multi-pathway,which could provide theoretical references for the study on the decoction.关键词
糖尿病足/四妙勇安汤/网络药理学/作用机制/信号通路Key words
diabetic foot/Simiaoyong'an decoction/network pharmacology/mechanism/signaling pathway分类
医药卫生引用本文复制引用
程亚清,牛少辉,曹乾,常金霞,方毅娜,王唱,白怡敏,曹建春..基于网络药理学探讨四妙勇安汤治疗糖尿病足的作用机制[J].西部中医药,2025,38(1):73-79,7.基金项目
国家自然科学基金面上项目(81874473). （81874473）
