广西师范大学学报（自然科学版）2025，Vol.43Issue(1)：31-38,8.DOI:10.16088/j.issn.1001-6600.2024042502
鸡屎藤内生真菌活性菌株筛选及其抗菌活性物质研究
Study on the Antifungal Activity of Secondary Metabolites from Active Endophytic Fungi Isolated from Paederia scandens(Lour.)Merr.
摘要
Abstract
The antifungal activities of different solvent extracts from fermentation products of 5 endophytic fungi isolated from Paederia scandens(Lour.)Merr.were determined by a mycelium growth rate method.The optimal active strain and its extraction layers were screened out,and the virulence was further determined.Based on the bioactivity-guiding,the active compound was separated by silica gel column chromatography,and the structure of the compounds was identified by nuclear magnetic resonance(NMR)and gas chromatography-mass spectrometry(GC-MS).The antifungal effect of active compound was also evaluated.The results showed that the ethyl acetate extraction layer of strain PsJ3 had the best antifungal activity against the 7 tested plant pathogenic fungi,with the effective medium concentration(EC50)ranging from 0.086 9 to 1.104 3 g/L.Among them,there was the most significantantifungal effect on C.paradoxa with the EC50 value at 0.086 9 g/L,Followed by Alternaria oleracea and Pestalotiopsis theae with the EC50 values at 0.208 2 and 0.239 7 g/L.It had the lowest antifugal effect on Phytophthora parasitica var with the EC50 value at 1.104 3 g/L.The active compound fusaric acid was obtained from the ethyl acetate layer of the strain PsJ3.It showed significant antifungal activity against all the above seven plant pathogenic fungi,with EC50 values ranging from 0.018 0 to 0.390 5 g/L.Similarly,it had the best antifungal effect against C.paradoxa,with EC50 value of 0.0180 g/L,followed by A.oleracea and P.theae with the EC50 values of 0.030 3 and 0.038 2 g/L.The Antifungal effect on P.parasitica var was the worst,with the EC50 value at 0.390 5 g/L.The results showed that the endophytic fungus of Paederia scandens(Lour.)Merr.contained a broad spectrum antifungal activity of secondary metabolites against plant pathogenic fungi,and provided a reference for the application of endophytic fungi associated with Paederia scandens(Lour.)Merr.in agricultural antifungal activity.关键词
鸡屎藤/内生真菌/次生代谢产物/抑菌活性/镰刀菌酸Key words
Paederia scandens(Lour.)Merr./endophytic fungi/secondary metabolites/antifungal activity/fusaric acid分类
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徐紫薇,邓业成,骆海玉,邓志勇,韦坚芬,黄玲玉,甘萍贵..鸡屎藤内生真菌活性菌株筛选及其抗菌活性物质研究[J].广西师范大学学报（自然科学版）,2025,43(1):31-38,8.基金项目
广西重点研发计划项目(桂科AB1850025) （桂科AB1850025）
桂林市重点研发计划项目(20190211-19) （20190211-19）
