海南医科大学学报2025，Vol.31Issue(3)：161-168,8.DOI:10.13210/j.cnki.jhmu.20240815.001
抗生素饲喂小鼠对发热伴血小板减少综合征病毒在蜱虫共吸血传播中的影响
Effect of antibiotic feeding in mice on the co-feeding transmission of fever with thrombocytopenia syndrome virus in ticks
摘要
Abstract
Objective:To investigate the role of mouse gut microbiota in the transmission of severe fever with thrombocytope-nia syndrome virus(SFTSV),a newly emerging tick-borne pathogen.Methods:The gut microbiota of Kunming mice was manip-ulated by antibiotics treatment and microbial recolonization.Then,infected adults and uninfected nymphs were co-fed on the mice.The SFTSV levels were determined in the engorged nymphs using quantitative real-time PCR(qPCR)analysis.Results:The al-teration of gut bacteria was confirmed by plating the homogenate of faeces onto Luria-Bcrtani(LB)agar plates.Microbial commu-nity displayed limited variety in the antibiotic treatment group compared to the control group and the recolonization group.In the model of co-feeding transmission,qPCR analysis revealed that the viral load increased in the antibiotic treatment group compared to the control group and the recolonization group.Conclusion:Mouse gut microbiota can inhibit the transmission of SFTSV.The results provide a basis for further exploration of functional bacteria of host gut in SFTSV transmission.关键词
严重发热伴血小板减少综合征病毒/长角血蜱/肠道/微生物Key words
Severe fever with thrombocytopenia syndrome virus/Haemaphysalis longicornis/Gut/Microbiota分类
医药卫生引用本文复制引用
陈京晶,陈晨,周祥,袁传飞,夏乾峰..抗生素饲喂小鼠对发热伴血小板减少综合征病毒在蜱虫共吸血传播中的影响[J].海南医科大学学报,2025,31(3):161-168,8.基金项目
This study was supported by the National Natural Science Foundation of China(82102433) 国家自然科学基金项目(82102433) （82102433）
