海南医科大学学报2025，Vol.31Issue(3)：200-210,11.DOI:10.13210/j.cnki.jhmu.20240925.002
葛花作用于Casp3调控细胞自噬干预肝癌的机制研究
Mechanism of Pueraria lobata in regulating liver cancer via Casp3-mediated cellular autophagy
摘要
Abstract
Objective:To investigate the mechanism of intervention of Pueraria lobata in hepatocellular carcinoma by cellular autophagy based on network pharmacology and experimental validation.Methods:The effects of medicated serum of Pueraria lo-bata on HepG2 cells were explored by cell proliferation and scratch experiments.The TCMSP database was used to obtain the tar-get of Pueraria lobata,the target of liver cancer disease from OMIM and other databases,and the autophagy gene of cells from the HADb database,and the protein interaction,GO and KEGG enrichment analysis were carried out after the intersection of the three targets,and the top 15 targets of Degree were screened.The 15 screened targets were analyzed by differential expression,ROC curves,clinical relevance,survival prognosis,molecular docking and molecular dynamics simulation.The final screened tar-gets were verified by Western blot,and the expression level of LC3B in different doses was detected.Results:Pueraria lobata medicated serum could significantly inhibit the proliferation and migration of HepG2 cells.The most important target of Pueraria lobata against liver cancer was Casp3,which can be stably bound to quercetin,the active ingredient of Pueraria lobata.Compared with the HepG2 control group,the expression of Casp3 protein in the Pueraria lobata sera group was significantly down-regulated,and LC3BII/LC3BI was significantly increased.Conclusion:Pueraria lobata can intervene in liver cancer via Casp3 mediated cellular autophagy.关键词
肝癌/葛花/细胞自噬/Casp3Key words
Liver cancer/Pueraria lobata/Cellular autophagy/Casp3分类
医药卫生引用本文复制引用
李军,成果,楼迪栋,张楠楠..葛花作用于Casp3调控细胞自噬干预肝癌的机制研究[J].海南医科大学学报,2025,31(3):200-210,11.基金项目
This study was supported by Natural Science Project of Guizhou Provincial Basic Research Program[Qiankehe Foundation ZK(2024)General 382] （2024）
Guizhou Provincial Administration Project in 2024(QZYY-2024-001) （QZYY-2024-001）
2023 Guizhou University of Traditional Chinese Medicine Graduate Education Innovation Plan Project(YCXKYB2023001) 贵州省基础研究计划自然科学项目[黔科合基础-ZK(2024)一般382] （YCXKYB2023001）
2024年贵州省中管局项目(QZYY-2024-001) （QZYY-2024-001）
2023年贵州中医药大学研究生教育创新计划项目(YCXKYB2023001) （YCXKYB2023001）
