海南医科大学学报2025，Vol.31Issue(4)：248-253,6.DOI:10.13210/j.cnki.jhmu.20240914.001
利福平通过NRF2/NQO1通路对小鼠肝细胞造成氧化损伤
Oxidative damage to mouse liver cells caused by rifampicin through the NRF2/NQO1 pathway
摘要
Abstract
Objective:To explore the effect of rifampicin regulating NRF2/NQO1 pathway on AML12 in mouse hepatocytes.Methods:Normal mouse liver cell line AML12 cells were cultured in this study.The cells were treated with various concentra-tions of rifampicin,and cell viability was assessed by CCK8 method.Based on the CCK8 results,suitable concentration of rifampi-cin were determined to be 50,100 and 200 µmol/L.The activity of catalase,superoxide dismutase(SOD)and the levels of malo-ndialdehyde were detected using the microporous plate method.The levels of intracellular reactive oxygen species and the mito-chondrial membrane potential were assessed by DCFH-DA and JC-1 fluorescence probe respectively using flow cytometry.The mRNA expression levels of NQO1,SOD and NRF2 genes were determined by qRT-PCR,and the protein expression levels of NRF2 and NQO1 were detected by Western Blot.Results:The activity of AML12 cells decreased with the increase of rifampicin concentration.Compared with the control group,the malondialdehyde content,catalase activity and reactive oxygen species levels of AML12 cells were increased in treated groups,as well as the mitochondrial membrane potential(P<0.05).The SOD activity and the mRNA expression levels of NQO1,SOD and NRF2 genes all demonstrated a significant decrease in response to increas-ing concentrations of Rifampicin(P<0.05).Consequently,the expression levels of NQO1 and NRF2 exhibited a similar trend.Conclusion:Rifampicin can cause oxidative damages through NRF2/NQO1 pathway by a concentration-dependent manner in mouse hepatocyte AML12 cell line.关键词
利福平/AML12细胞/氧化损伤Key words
Rifampin/AML12 hepatocytes/Oxidative damage分类
医药卫生引用本文复制引用
李亚奇,郑志坚,张美诗,符王洋,孟春燕,钱庆增..利福平通过NRF2/NQO1通路对小鼠肝细胞造成氧化损伤[J].海南医科大学学报,2025,31(4):248-253,6.基金项目
This study was supported by the Basic Scientific Research Projects Expenses of Colleges and Universities in Hebei Province(JQN2021032)河北省高等学校基本科研业务费(JQN2021032) （JQN2021032）
