海南医科大学学报2025，Vol.31Issue(4)：254-263,10.DOI:10.13210/j.cnki.jhmu.20240914.004
周末勇士运动抑制炎症和铁死亡减轻β-肾上腺素能超载诱导的心脏损伤
Weekend warrior exercise reduces β-adrenergic overload induced cardiac injury by inhibition of inflammation and ferroptosis
摘要
Abstract
Objective:To explore the effect and possible mechanism of weekend warrior exercise(WWE)on β-adrenergic overload induced cardiac injury.Methods:The mice were randomly divided into Con group,ISO group and WWE+ISO group.After 4-weeks weekend training,the mouse cardiac injury model was established by subcutaneous injection of ISO into the neck.After 3 days,HMI,serum CK-MB and cTnI concentration were measured.HE staining and Sirius red staining were used to ob-serve the degree of cardiac injury and fibrosis.TUNEL staining was used to observe myocardial cell apoptosis.The qRT-PCR,Western blot and immunofluorescence staining were used to detect the expression of genes related to cardiac fibrosis and hypertro-phy,the levels of inflammation related cytokines and the expression of ferroptosis related genes and proteins in mice.Results:Compared with ISO group,HMI value and CK-MB concentration in WWE+ISO group significantly decreased(P<0.05),and cTnI concentration showed a downward trend(P>0.05).Compared with ISO group,WWE+ISO group had a more orderly ar-rangement of myocardial cells and a significant decrease in inflammatory cell infiltration.The area of cardiac fibrosis in WWE+ISO group was significantly lower than that in ISO group(P<0.01).Compared with ISO group,the mRNA levels of Col 1,Col 3 and Anp in WWE+ISO group were significantly reduced(P<0.05).The number of TUNEL positive cells in WWE+ISO group was significantly lower than that in ISO group(P<0.05).Compared with ISO group,the mRNA levels of IL-6,Tnf-α and IL-1β were significantly reduced in WWE+ISO group(P<0.05).The mRNA levels of Nrf2 and GPX4 protein expression in WWE+ISO group were significantly higher than those in ISO group(P<0.05),the mRNA and protein expression of HO-1 in WWE+ISO group were significantly lower than those in ISO group(P<0.05).The number of HO-1 fluorescent positive cells in WWE+ISO group was significantly lower than that in ISO group(P<0.05).Conclusion:Weekend warrior exercise can reduce β-adrenergic overload induced cardiac injury,the mechanism may be related to the inhibition of cardiac inflammation and ferropto-sis.关键词
周末勇士运动/异丙肾上腺素/心脏损伤/炎症/铁死亡Key words
WWE/ISO/cardiac injury/inflammation/ferroptosis分类
社会科学引用本文复制引用
秦娣,秦学林,郑一苇,林毅,彭勇..周末勇士运动抑制炎症和铁死亡减轻β-肾上腺素能超载诱导的心脏损伤[J].海南医科大学学报,2025,31(4):254-263,10.基金项目
This study was supported by Young and Middle-aged Academic Leaders of"Blue Project"in Jiangsu Province （）
National Level Project of Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province(202310330003Z) （202310330003Z）
"1+1"Excellent Academic Team Project of Nanjing Sport Institute(XSTD202317)江苏省高校"青蓝工程"中青年学术带头人项目 （XSTD202317）
江苏省高等学校大学生创新创业训练计划国家级项目(202310330003Z) （202310330003Z）
南京体育学院"1+1"优秀学术团队项目(XSTD202317) （XSTD202317）
