海南医科大学学报2025，Vol.31Issue(4)：264-269,6.DOI:10.13210/j.cnki.jhmu.20240624.001
氧化克班宁促进人乳腺癌MCF-7细胞微管解聚的机制研究
Study on the mechanisms of promoting microtubule depolymerization by oxocrebanine in human breast cancer MCF-7 Cells
摘要
Abstract
Objective:This paper explores the mechanism of oxocrebanine in promoting microtubule depolymerization of hu-man breast cancer MCF-7 cells through the Rho/ROCK signaling pathway.Methods:Firstly,immunofluorescence was used to study the effect of oxocrebanine on F-actin,a key protein in the skeleton of MCF-7 cells.Secondly,the Western Blot method was used to detect the protein expression levels of F-actin and Rho A,ROCK1 and ROCK2,key proteins of the Rho/ROCK signaling pathway.At last,the phosphorylation level of Rho/ROCK downstream proteins MYPT and LIMNK were detected by Western Blot method while MCF-7 cells were treated with GSK429286A and oxocrebanine.Results:After MCF-7 cells were treated with oxocrebanine for 48 h,the fluorescence intensity of F-actin protein gradually decreased,the expression level of F-actin protein was also significantly reduced(P<0.01),and the expression levels of Rho A,ROCK1 and ROCK2 protein were significantly reduced(P<0.01).After MCF-7 cells were treated with GSK429286A and oxocrebanine,the phosphorylation levels of MYPT and LIMK,Rho/ROCK downstream proteins,were reduced in a dose-dependent manner(P<0.01).Conclusion:Oxocrebanine can destroy the cytoskeleton,interfere in the Rho/ROCK pathway,regulate the phosphorylation levels of its downstream proteins MYPT and LIMK,promote microtubule depolymerization and inhibit human breast cancer MCF-7 cells growth.关键词
海南地不容/氧化克班宁/MCF-7细胞/微管/Rho/ROCK通路Key words
Stephania hainanensis H.S.Lo et Y.Tsoong/Oxocrebanine/MCF-7 cell line/Microtubule/Rho/ROCK path-way分类
医药卫生引用本文复制引用
于蕾,闫彩凤,宋辉,郎朗,于晶涵,马瑗晗,王正文..氧化克班宁促进人乳腺癌MCF-7细胞微管解聚的机制研究[J].海南医科大学学报,2025,31(4):264-269,6.基金项目
Hainan Provincial Natural Science Foundation Project(820RC776) （820RC776）
Hainan Province Health Technology Innovation Joint Project(WSJK2024MS162) （WSJK2024MS162）
2021 Harbin Business University Teacher"Innovation"Project Support Program Project(LH2020H070) （LH2020H070）
Heilongjiang Province Traditional Chinese Medicine Research Project(ZHY2024-098)海南省自然科学基金项目(820RC776) （ZHY2024-098）
海南省卫生健康科技创新联合项目(WSJK2024MS162) （WSJK2024MS162）
2021年哈尔滨商业大学教师"创新"项目支持计划项目(LH2020H070) （LH2020H070）
黑龙江省中医药科研课题项目(ZHY2024-098) （ZHY2024-098）
