海南医科大学学报2025，Vol.31Issue(4)：270-276,7.DOI:10.13210/j.cnki.jhmu.20240912.002
血清iFABP和DAO对脓毒症及脓毒症肠道损伤的诊断及预后评判价值
Diagnostic and prognostic value of serum iFABP and DAO on sepsis and intestinal barrier dysfunction with sepsis
摘要
Abstract
Objective:To investigate the relationship between intestinal barrier dysfunction and the levels of enteric fatty acid binding protein(iFABP)and diamine oxidase(DAO)in peripheral blood of patients with sepsis.Methods:Sepsis patients(n=129)and healthy subjects(n=129)were selected as subjects,and the expression levels of DAO and iFABP in peripheral blood were detected by ELISA.According to AGI score,sepsis patients were divided into a sepsis AGI group and a sepsis non-AGI group,and DAO and iFABP expression levels were compared between the two groups.Logistic regression was used to analyze the relationship between DAO and iFABP expression levels in peripheral blood and intestinal dysfunction.Patients were divided in-to a survival group(n=93)and a death group(n=36)according to their survival status at 28 d.Cox risk regression analysis was used to analyze the risk factors affecting the prognosis of patients with sepsis,and the working characteristic curve of subjects was further drawn to evaluate the expression levels of peripheral blood DAO and iFABP in patients with sepsis.Results:The expres-sion levels of peripheral blood DAO and iFABP of patients with sepsis were higher than those of the healthy control group,and the expression levels of peripheral blood DAO and iFABP of patients with sepsis in the AGI group were higher than those in the non-AGI group.Logistic regression analysis showed that the OR value between iFABP and intestinal dysfunction was 1.075,and that between DAO and intestinal dysfunction was 1.196.ROC curve analysis showed that when iFABP cut-off value was 410.178,AUC was 0.877(95％CI:0.812-0.942),sensitivity was 84.90％,and specificity was 82.90％.When DAO truncation value was 3.473,AUC was 0.606(95％CI:0.500-0.712),sensitivity was 64.20％,and specificity was 68.40％.Cox multivariate analysis indicated that DAO and iFABP levels in peripheral blood and APACHE Ⅱ score were independent risk factors affecting the prog-nosis of sepsis patients.The area under ROC curve for the prognosis of patients assessed by DAO was 0.985,and its truncation value was 3.99,sensitivity was 97.2％,and specificity was 92.5％.The area under ROC curve for iFABP to evaluate the progno-sis of patients was 0.654,and its cut-off value was 418.36,sensitivity was 61.1％,and specificity was 84.9％.Conclusions:The expression levels of peripheral blood DAO and iFABP of patients with sepsis are significantly increased,which is closely related to intestinal damage caused by sepsis.Meanwhile,the expression levels of peripheral blood DAO and iFABP are closely related to the prognosis of sepsis patients.关键词
脓毒症/肠道损伤/肠道脂肪酸结合蛋白((FABP)/二胺氧化酶(DAO)Key words
Sepsis/Intestinal injury/Intestinal fatty acid binding protein(iFABP)/Diamine oxidase(DAO)分类
医药卫生引用本文复制引用
李志华,于湘友,王毅,银乐乐..血清iFABP和DAO对脓毒症及脓毒症肠道损伤的诊断及预后评判价值[J].海南医科大学学报,2025,31(4):270-276,7.基金项目
This study was supported by the National Natural Science Foundation of China(82160360)国家自然科学基金资助项目(82160360) （82160360）
