华东理工大学学报（自然科学版）2025，Vol.51Issue(1)：50-59,10.DOI:10.14135/j.cnki.1006-3080.20240121001
延长盐单胞菌生产依克多因高密度发酵工艺优化
High-Density Fermentation Process Optimization for Ectoine production by Halomonas elongata DSM 2581T
摘要
Abstract
In this study,a high-density fermentation process of Halomonas elongate DSM 2581T was established,which had the advantage of high ectoine production rate.The effects of nutrient components of defined medium on fermentation and metabolism of H.elongata DSM 2581T were investigated by single factor experiment,Plackett-Burman and Central Composite Design.The results showed that sodium citrate(3 g/L),NH4Cl(3 g/L)and NaCl(80 g/L)could significantly promote the growth and ectoine production of H.elongata DSM 2581T.Furthermore,according to the growth and metabolism of H.elongata in the defined medium in a 5 L fermentor,the feeding mode of glucose continuous flow plus and pH feedback control(ammonia water)was established to carry out the high-density fermentation process of H.elongata DSM 2581T.Salt shock,which increased the NaCl concentration in the fermentation broth from 80 g/L to 130 g/L in the exponential growth phase,resulted in OD600 reaching 169.3 after 13 h of fermentation,with ectoine production and ectoine synthesis reaching 20 g/L and 1.54 g/(L·h),respectively.This study provides theoretical guidance for the low-cost and efficient industrial ectoine production.关键词
延长盐单胞菌/合成培养基/高细胞密度培养/依克多因/盐冲击Key words
Halomonas elongata/defined medium/high cell density culture/ectoine/salt shock分类
生物科学引用本文复制引用
张悦,刘昊,刘玮,余君雄,肖扬,郇晶伟,颜诚旭,庄英萍,王泽建..延长盐单胞菌生产依克多因高密度发酵工艺优化[J].华东理工大学学报（自然科学版）,2025,51(1):50-59,10.基金项目
国家重大专项项目(2020YFA096800) （2020YFA096800）
