湖南中医药大学学报2025，Vol.45Issue(1)：23-29,7.DOI:10.3969/j.issn.1674-070X.2025.01.004
从肝治心组方对心肌缺血再灌注损伤大鼠心肌Nrf2、HO-1和铁转运相关蛋白的影响
Effects of Conggan Zhixin Formula on Nrf2,HO-1,and iron transport-related proteins in myocardial ischemia-reperfusion injury in rats
摘要
Abstract
Objective To study the myocardial protective effects of Conggan Zhixin Formula on myocardial ischemia-reperfusion injury(MIRI)in rats and its influence on the expressions of nuclear factor-erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),divalent metal transporter 1(DMT1),and hephaestin(Heph).Methods Sixty male SPF-grade SD rats were randomly divided into normal group,sham-operated group,model group,Conggan Zhixin Formula group[5.32 g/(kg·d)],Shexiang Baoxin Pill group[10.27 mg/(kg·d)],and diltiazem group[6.86 mg/(kg·d)],with ten rats in each group.A MIRI rat model was established by ligating the left anterior descending coronary artery for 30 minutes followed by 120 minutes of reperfusion.Samples were collected after 14 consecutive days of gavage administration post-surgery.HE staining was used to observe the morphological changes in myocardial tissue,Prussian blue staining was employed to assess iron deposition in the myocardial tissue,and transmission electron microscopy was utilized to examine the ultrastructure of myocardial tissue.RT-PCR and Western blot were performed to examine the mRNA and protein expressions of Nrf2,HO-1,DMT1,and Heph.Results Compared with the normal group and sham-operated group,the model group exhibited disordered arrangement of myocardial fibers,fibrous scar tissue hyperplasia,high levels of iron deposition,abnormal mitochondrial structure with blurred cristae,and downregulated mRNA and protein expressions of Nrf2,HO-1,and Heph(P＜0.05),as well as upregulated mRNA and protein expression of DMT1(P＜0.05).Compared with the model group,the rats in each drug-administered group showed reduced morphological damage in the myocardial tissue,reduced levels of iron deposition,more intact mitochondrial and cristae structures,more intact mitochondrial and cristae structures,upregulated mRNA and protein expressions of Nrf2,HO-1,and Heph(P＜0.05),and downregulated mRNA and protein expression of DMT1(P＜0.05).Conclusion Conggan Zhixin Formula may exert its effects on reducing MIRI by activating the Nrf2/HO-1 signaling pathway,upregulating Heph expression,downregulating DMT1 expression,and reducing iron deposition in myoc-ardial tissue.关键词
心肌缺血再灌注损伤/从肝治心组方/核因子E2相关因子2/血红素加氧酶-1/膜铁转运辅助蛋白/二价金属转运蛋白1/铁代谢失衡Key words
myocardial ischemia-reperfusion injury/Conggan Zhixin Formula/nuclear factor-erythroid 2-related factor 2/heme oxygenase-1/membrane iron transporter accessory protein/divalent metal transporter 1/iron metabolism imbalance分类
医药卫生引用本文复制引用
汪辛强,何飘,朴美虹,谢丽华,曾阳,王瑾茜,张程程,胡国恒,陈亚..从肝治心组方对心肌缺血再灌注损伤大鼠心肌Nrf2、HO-1和铁转运相关蛋白的影响[J].湖南中医药大学学报,2025,45(1):23-29,7.基金项目
全国名老中医药专家传承工作室建设项目(国中医药函人教函[2022]75号) （国中医药函人教函[2022]75号）
湖南省自然科学基金项目(2021JJ40418) （2021JJ40418）
湖南省卫生健康委员会科研计划项目(202203074417) （202203074417）
湖南中医药大学第一附属医院中医药传承创新专项(2024XYLH363) （2024XYLH363）
湖南中医药大学校级科研项目(Z2023XJYB25) （Z2023XJYB25）
湖南中医药大学中医学世界一流培育学科(2023). （2023）
