湖南中医药大学学报2025，Vol.45Issue(1)：30-38,9.DOI:10.3969/j.issn.1674-070X.2025.01.005
基于Keap1/Nrf2信号通路探讨旋覆代赭汤含药血清对食管癌细胞的调控作用
Regulatory effects of Xuanfu Daizhe Decoction-medicated serum on esophageal cancer cells based on Keap1/Nrf2 signaling pathway
摘要
Abstract
Objective To investigate the regulatory effects of Xuanfu Daizhe Decoction(XFDZD)-medicated serum on esophageal cancer cells.Methods Human esophageal cancer TE-1 and TE-10 cells were subjected to primary culture for seven days,followed by treatment with different concentrations of XFDZD-medicated serum(0%,5%,10%,15%,20%,25%,and 30%)and different concentrations of polyene paclitaxel(0,0.1,1,5,10,and 50 nmol/L)for 48 hours.The CCK-8 experiment was used to screen the optimal administration concentration of XFDZD-medicated serum.TE-1 and TE-10 cells were randomly divided into model,blank serum,medicated serum,and polyene paclitaxel groups.After grouping,the CCK-8 assay was used to determine cell proliferation ability.The Transwell assay was used to measure cell migration and invasion abilities.Flow cytometry and Tunnel assay were conducted to examine cell apoptosis,and immunofluorescence was used to determine the protein expression levels of Kelch-like ECH-associated protein 1(Keap1)and nuclear factor-erythroid 2-related factor 2(Nrf2).Results The optimal administration concentration of XFDZD-medicated serum was 30%,and the optimal concentration of polyene paclitaxel was 1 nmol/L.Compared with the model group and the blank serum group,the proliferation,migration,invasion abilities,and the protein expression levels of Keap1 and Nrf2 of TE-1 and TE-10 cells were reduced in both the medicated serum group and the polyene paclitaxel group(P＜0.05),while the cell apoptosis rates were elevated(P＜0.05).Compared with polyene paclitaxel group,the proliferation ability of TE-1 and TE-10 cells,the protein expression of Keap1 in TE-1 cells,and the protein expressions of Keap1 and Nrf2 in TE-10 cells were elevated in the medicated serum group(P＜0.05),whereas the apoptosis rates of TE-1 cells was reduced(P＜0.05).Conclusion XFDZD-medicated serum may inhibit the proliferation of esophageal cancer cells by regulating the Keap1/Nrf2 signaling pathway.关键词
食管癌/旋覆代赭汤/多西他赛/Keap1/Nrf2通路/增殖/凋亡Key words
esophageal cancer/Xuanfu Daizhe Decoction/polyene paclitaxel/Keap1/Nrf2 pathway/proliferation/apoptosis分类
医药卫生引用本文复制引用
田艳鹏,陈卫卫,王海峰,赵泽鹏,乔柱,刘善军..基于Keap1/Nrf2信号通路探讨旋覆代赭汤含药血清对食管癌细胞的调控作用[J].湖南中医药大学学报,2025,45(1):30-38,9.基金项目
济宁市重点研发计划项目(2023YXNS256) （2023YXNS256）
济宁医学院附属医院博士专项科研基金项目(2021-BS-020). （2021-BS-020）
