湖南中医药大学学报2025，Vol.45Issue(1)：39-48,10.DOI:10.3969/j.issn.1674-070X.2025.01.006
基于网络药理学探讨扶正解毒抗癌汤治疗黑色素瘤的作用机制
Mechanism of action of Fuzheng Jiedu Kang'ai Decoction in treating melanoma based on network pharmacology
摘要
Abstract
Objective To explore the molecular mechanism of Fuzheng Jiedu Kang'ai Decoction(FZJDD)in the treatment of melanoma.Methods The effective chemical components of FZJDD and their corresponding targets were retrieved from the databases and literature of TCMSP and TCMID.Melanoma-related targets were screened using databases such as GeneCard,OMIM,and TTD to obtain intersection targets of"drug-disease".Protein-protein interaction(PPI)network analysis was performed using STRING11.5 and DAVID databases to identify core targets.Cytoscape 3.8.1 was used to construct a Chinese medicines-action targets-pathway networks for FZJDD in treating melanoma.Gene ontology function(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were conducted to identify the key biological processes and signaling pathways involved in treating melanoma with FZJDD.Quantitative real-time PCR(qPCR)and Western blot were used to validate the roles of key proteins in related signaling pathways.Results A total of 47 active components of FZJDD and 168"drug-disease"intersection targets were identified.The enriched sign aling pathways mainly included phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway,among others.In vitro cell experiments showed that FZJDD effectively inhibited the proliferation of melanoma A375 cells and suppressed the protein and mRNA expression levels of key proteins in PI3K/AKT signaling pathway,including PI3K,AKT,and the mammalian target of rapamycin(mTOR)(P＜0.05).Conclusion FZJDD can treat melanoma by exerting anti-inflammatory effects and promoting tumor cell apoptosis through multiple components,targets,and pathways,among which the regulation of the PI3K/AKT pathway may be its key mechanism.关键词
扶正解毒抗癌汤/黑色素瘤/PI3K/AKT信号通路/网络药理学/作用机制Key words
Fuzheng Jiedu Kang'ai Decoction/melanoma/PI3K/AKT signaling pathway/network pharmacology/mecha-nism of action分类
医药卫生引用本文复制引用
涂雅玲,陈其华,席建元,祁林,赵多多,喻环宇..基于网络药理学探讨扶正解毒抗癌汤治疗黑色素瘤的作用机制[J].湖南中医药大学学报,2025,45(1):39-48,10.基金项目
湖南省研究生科研创新重点项目(CX20210680) （CX20210680）
湖南省教育厅科学研究重点项目(21A0224) （21A0224）
湖南省教育厅科学研究优秀青年项目(23B0377) （23B0377）
湖南省中医药管理局中医药科研课题(C2024012) （C2024012）
2024年湖南省大学生创新训练计划项目(S202410541030) （S202410541030）
湖南中医药大学校院联合基金重点项目(2023XYLH002). （2023XYLH002）
