湖南中医药大学学报2025，Vol.45Issue(1)：49-58,10.DOI:10.3969/j.issn.1674-070X.2025.01.007
基于数据挖掘和动物实验探讨含桔梗方剂的用药规律及作用机制
Exploring the medication patterns and mechanism of action of Jiegeng(Platycodonis Radix)-containing formulas based on data mining and animal experiments
摘要
Abstract
Objective To elucidate the combination rules with other Chinese medicines,underlying mechanism of action,and pharmacological effects of Jiegeng(Platycodonis Radix)through the integrated use of data mining,network pharmacology,and animal experimentation,thereby providing a scientific basis for its application in clinical treatment and research and development.Methods Data on formulas containing Jiegeng(Platycodonis Radix)were collected from the Traditional Chinese Medicine Formula Database of the Drug Intelligence Network.Statistical analysis methods such as frequency,association rules,and clustering were applied for data mining,and data visualization was conducted using Cytoscape 3.8.0 software.Theraputic targets of Jiegeng(Platycodonis Radix)obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database,integrated with the disease targets of pneumonia from the GeneCards and Online Mendelian Inheritance in Man(OMIM)databases,were used to construct a protein-protein interaction(PPI)network.Subsequently,GO and KEGG enrichment analyses were performed.Furthermore,an acute pneumonia model was established using lipopolysaccharide(LPS)to assess the efficacy of Jiegeng(Platycodonis Radix)in treating pneumonia.Results A total of 1,811 formulas containing Jiegeng(Platycodonis Radix)were included,involving 484 types of Chinese medicines with a cumulative frequency of 22,155 administrations.Jiegeng(Platycodonis Radix)was commonly combined with tonic medicines,phlegm-transforming,cough-stopping,and panting-alleviating medicines,exterior-releasing medicines,heat-clearing medicines,and qi-regulating medicines,which were mainly warm,cold,or neutral in property and pungent or bitter in flavor,and primarily entered the lung,spleen,stomach,and heart meridians.Association rule analysis revealed seven core Chinese medicine combinations with a support rate greater than 25%.Cluster analysis identified high-frequency Chinese medicines with a frequency greater than 300,resulting in three three-medicine combinations,one two-medicine combination,and three single Chinese medicines.The main indications were disorders of lung system,qi,blood,and body fluids,and spleen and stomach system,with the highest frequency in disorders of lung system.In the treatment of lung system disorders,cluster analysis yielded two three-medicine combinations and three single Chinese medicines.Network pharmacology analysis showed that the main active ingredients of Jiegeng(Platycodonis Radix),including acacetin,cis-dihydroquercetin,and luteolin,can modulate multiple pathways and targets to alleviate pneumonia.Results of the animal experiments confirmed that Jiegeng(Platycodonis Radix)significantly reduced lung tissue structural damage and the secretion levels of inflammatory factors in serum induced by LPS(P＜0.05).Conclusion As a commonly used Chinese medicine in clinical practice,especially in the treatment of lung system diseases,Jiegeng(Platycodonis Radix)can exert therapeutic effects through various pathways when combined with a range of other Chinese medicines.关键词
桔梗/数据挖掘/配伍规律/网络药理学/肺炎/动物实验/药效Key words
Jiegeng(Platycodonis Radix)/data mining/combination rule/network pharmacology/pneumonia/animal ex-periments/pharmacodynamics分类
医药卫生引用本文复制引用
黄家望,付靖敏,冯芷莹,王康宇,刘卓琳,蒋雪莲,杨佳欣,喻嵘,李玲..基于数据挖掘和动物实验探讨含桔梗方剂的用药规律及作用机制[J].湖南中医药大学学报,2025,45(1):49-58,10.基金项目
国家自然科学基金资助项目(81973670) （81973670）
湖南省教育厅优秀青年项目(23B0379) （23B0379）
湖南省中医药科研课题重点项目(A2024010) （A2024010）
长沙市自然科学基金项目(kq2402183). （kq2402183）
