湖南中医药大学学报2025，Vol.45Issue(1)：59-64,6.DOI:10.3969/j.issn.1674-070X.2025.01.008
整合指纹图谱与多成分含量测定的益肾固精暖脐贴质量评价研究
Quality evaluation of Yishen Gujing Nuanqi Patch through integration of fingerprinting and multi-component content determination
摘要
Abstract
Objective To establish a high performance liquid chromatography(HPLC)fingerprint for Yishen Gujing Nuanqi Patch(YSGJNQP)and to determine the content of six components(cinnamic aldehyde,eugenol,hydroxy-ɑ-sanshool,hydroxy-β-sanshool,parent eugenol,and osthole)in different batches,so as to provide the reference for the quality control of YSGJNQP.Methods Fingerprints of 15 batches of YSGJNQP were established by HPLC.The similarity evaluation and orthogonal partial least square method-discriminant analysis(OPLS-DA)were used to screen out the differential components,and the content of six index components was determined.Results The HPLC fingerprint of YSGJNQP and the content determination method of the six index components were established.The similarity among 15 batches of samples ranged from 0.874 to 0.999,twenty-one common peaks and six chromatographic peaks were identified,and 12 differential components were screened out.The content of the six index components was found to be stable across the 15 batches of YSGJNQP.Conclusion The established HPLC fingerprint of YSGJNQP and the content determination method of its six components are accurate,stable,and reproducible.Consequently,it can be effectively used for the quality control of YSGJNQP.关键词
益肾固精暖脐贴/指纹图谱/含量测定/质量评价/相似度评价/正交偏最小二乘法-判别分析Key words
Yishen Gujing Nuanqi Patch/fingerprint/content determination/quality evaluation/similarity evaluation/or-thogonal partial least square method-discriminant analysis分类
医药卫生引用本文复制引用
廖卓,包晴,高元航,李海英,王璐,唐林,杨磊..整合指纹图谱与多成分含量测定的益肾固精暖脐贴质量评价研究[J].湖南中医药大学学报,2025,45(1):59-64,6.基金项目
湖南省自然科学基金项目(2022JJ80094,2024JJ6362) （2022JJ80094,2024JJ6362）
湖南省卫生健康高层次人才项目(湘卫函[2024]43号). （湘卫函[2024]43号）
