湖南中医药大学学报2025，Vol.45Issue(1)：65-72,8.DOI:10.3969/j.issn.1674-070X.2025.01.009
电针内关、膻中穴改善慢性心力衰竭模型大鼠的症状及代谢组学特征
On the improvements of electroacupuncture at"Neiguan"(PC6)and"Danzhong"(CV17)acupoints on symptoms and metabolomic characteristics in a rat model of chronic heart failure
摘要
Abstract
Objective To explore the effects of electroacupuncture(EA)at"Neiguan"(PC6)and"Danzhong"(CV17)acupoints on symptoms and metabolomic characteristics in rats with chronic heart failure(CHF).Methods After seven days of adaptive feeding,a CHF model was established in SD rats by intraperitoneal injections of doxorubicin(DOX)on alternate days.The successfully modeled CHF rats were randomized using a random number table into model group(n=11),EA group(n=14),and blank control group(n=11)Rats in the EA group received EA intervention at"Neiguan"(PC6)and"Danzhong"(CV17)acupoints once a day for two weeks,while the model and blank control groups only underwent routine restraint without intervention.At the end of the intervention,cardiac pumping function was evaluated by echocardiography,and pathological changes in left ventricular myocardial tissue of the rats in each group were assessed using H&E staining.LC-MS was used to check the metabolites in myocardial tissue of rats in each group.Principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)were conducted to screen out differential metabolites.Metabolic pathway analysis was performed using Metabo Analyst.Results Compared with the blank control group,the left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)in the model group significantly decreased(P＜0.01).After EA treatment,the LVEF and LVFS of echocardiogram in EA group were significantly increased compared with those in the model group(P＜0.01),indicating that EA can effectively improve the cardiac pumping function of CHF rats.Compared to the model group,the myocardial tissues showed reduced inflammatory infiltration,smoother muscle fibers,and more intact cell structures after intervention in the EA group,suggesting that EA can reverse myocardial damage caused by CHF.In the metabolomic test,compared with the blank control group,the model group revealed changes in three metabolites(P＜0.05)and four metabolic pathways in the myocardial tissues.After EA treatment,four metabolites showed changes(P＜0.05),with one metabolite significantly reversed.Metabolic pathway analysis indicated that EA can modulate purine metabolism,folate biosynthesis,and glutathione metabolism pathways.Conclusion EA at"Neiguan"(PC6)and"Danzhong"(CV17)acupoints significantly improves cardiac pumping function in CHF rats.The mechanism may be related to the regulation of myocardial energy metabolism,oxidative stress,and nucleotide synthesis.关键词
慢性心力衰竭/电针/内关/膻中/代谢组学/超声心动图Key words
chronic heart failure/electroacupuntcture/"Neiguan"(PC6)/"Danzhong"(CV17)/metabolomics/echocardiog-raphy分类
医药卫生引用本文复制引用
李祖强,丁攀婷,梁枝懿,周竞颖,刘琼,钟欢,刘密..电针内关、膻中穴改善慢性心力衰竭模型大鼠的症状及代谢组学特征[J].湖南中医药大学学报,2025,45(1):65-72,8.基金项目
国家自然科学基金项目(81904097) （81904097）
湖南省重点研发计划项目(2024JK2132) （2024JK2132）
国家中医药管理局2022年青年岐黄学者培养项目(国中医药人教函[2022]256号) （国中医药人教函[2022]256号）
湖南省自然科学基金项目(2020JJ5430) （2020JJ5430）
湖南省中医药管理局项目(B2023109). （B2023109）
