菌物学报2025，Vol.44Issue(2)：11-27,17.DOI:10.13346/j.mycosystema.240197
24种竹类植物根系丛枝菌根真菌群落组成及多样性差异分析
Differences in community composition and diversity of arbuscular mycorrhizal fungi in root systems of 24 bamboo species
摘要
Abstract
Bamboos are an important forest resource in China because of their significant economic and ecological values.Arbuscular mycorrhizal fungi(AMF)play an important role in bamboo growth and development.However,research on AMF communities associated with bamboo roots is extremely limited.This study was conducted in accordance with the data of National Bamboo and Flower Germplasm Collection,and focused on 24 bamboo species of eight genera in China,including Phyllostachys edulis and Ph.glauca.The Illumina MiSeq high-throughput sequencing platform was used to explore the effects of bamboo species identity and soil chemical properties on the species diversity and community composition of AMF.The relationship between structural heterogeneity of AMF community composition and genetic distance of bamboos was also analyzed.Additionally,a network analysis was performed to investigate the bipartite network structure characteristics of bamboo-AMF interactions.A total of 1 805 725 sequences of all the samples was detected,resulting in the acquirement of 473 amplicon sequence variants(ASVs)that were categorized in nine genera of which Glomus was dominant,and Scutellospora,Acaulospora and Gigaspora were common.In the sample plot,bamboos'identity had a significant individual effect on the differences in diversity indices(AMF Shannon-Weiner index and ASV richness)and the AMF community structure associated with the roots of different bamboo species.Bamboos'identity explained 22.28％of the total variation in diversity indices,20.06％of the total variation in the community structure at the ASV level,and 27.40％of the total variation in the community structure at the genus level.Six soil factors,including pH,did not contribute significantly to the differences in the root AMF community diversity and structure among bamboo species in the studied area.The genetic distance between different bamboo species was significantly positively correlated with the mean value of Hellinger distance of the root AMF community structure,indicating that more closely related bamboo species had more similar root AMF community structure.Structural features of bamboo-AMF interaction network cannot be predicted by null models of assuming species random interaction.The structure of this bipartite network was characterized by low level of nestedness(25.07),low level of connectance(0.17),low-to-medium level of specialization(0.35),and low-to-medium level of modularity(0.36),suggesting that there was a highly intense competition for resources among bamboo root-related AMF and that the interaction networks are relatively unstable.关键词
Illumina MiSeq测序/遗传距离/Hellinger距离/互作网络/嵌套性Key words
Illumina MiSeq sequencing/genetic distance/Hellinger distance/interaction network/nestedness引用本文复制引用
魏松坡,范少辉,刘广路..24种竹类植物根系丛枝菌根真菌群落组成及多样性差异分析[J].菌物学报,2025,44(2):11-27,17.基金项目
国际竹藤中心基本科研业务费专项(1632020019,1632021021)This work was supported by the Fundamental Research Funds for the International Centre for Bamboo and Rattan(1632020019,1632021021). （1632020019,1632021021）
