菌物学报2025，Vol.44Issue(2)：44-54,11.DOI:10.13346/j.mycosystema.240228
高卢蜜环菌共生猪苓多孔菌菌核际土壤的蛋白组研究Ⅰ
Sclerosphere soil proteome of Polyporus umbellatus during symbiosis with Armillaria gallica Ⅰ
摘要
Abstract
The exploration of expression and changes of sclerosphere soil proteome of Polyporus umbellatus during symbiosis with Armillaria gallica was conducted in Liuba County,Shaanxi Province.Four experimental groups were set up including blank control group(CK),non-symbiotic A.gallica group(CKA),non-symbiotic sclerotigenic P.umbellatus group(CKp)and experimental A.gallica and P.umbellatus simbiotic group(Tp+A).Proteomics analysis was carried out and high resolution mass spectrometry was applied to identify proteins from these groups.The results showed that the interaction between P.umbellatus and A.gallica could change the protein expression profile of each other in the sclerosphere.The informatics analysis indicated that the released protein of non-symbiotic A.gallica played catalytic and transferase activities,and participated in the phosphatidylinositol signaling system,GABA signal and other metabolic pathways;thioredoxin-like protein was specifically expressed by symbiotic A.gallica to exert antioxidant and effector activities.P.umbellatus sclerotium is not a complete resting structure,and can release enzymes involved in the metabolism of amino acids,taurine,phosphonate,etc.SNARE interactions in vesicular transport may play an important role in regulating the exchange and transport of substances between sclerotia and sclerosphere.After infection of A.gallica,sclerotia may adapt to symbiotic relationship through activated mechanosensitive ion channel and calcium signal transduction.This study provides valuable references for further research on mutualistic mechanisms of symbiosis between P.umbellatus and Armillaria sp.关键词
猪苓多孔菌/高卢蜜环菌/菌核/菌核际/蛋白质组Key words
Polyporus umbellatus/Armillaria gallica/sclerotia/sclerosphere/proteomics引用本文复制引用
李兵,周凌峰,刘柳,郭顺星..高卢蜜环菌共生猪苓多孔菌菌核际土壤的蛋白组研究Ⅰ[J].菌物学报,2025,44(2):44-54,11.基金项目
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031,2022-I2M-2-001) （2021-I2M-1-031,2022-I2M-2-001）
中央本级重大增减支项目"名贵中药资源可持续利用能力建设项目"(2060302)This work was supported by the Chinese Academy of Medical Science Innovation Fund for Medical Sciences(CIFMS,2021-I2M-1-031,2022-I2M-2-001)and the Key Project at Central Government Level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(2060302). （2060302）
