菌物学报2025，Vol.44Issue(2)：55-67,13.DOI:10.13346/j.mycosystema.240194
组蛋白赖氨酸甲基转移酶CfDot1在果生刺盘孢中的功能
Function of histone lysine methyltransferase CfDot1 in Colletotrichum fructicola
摘要
Abstract
Colletotrichum fructicola is the principal pathogen responsible for anthracnose in Camellia oleifera.Investigating histone lysine methyltransferase CfDot1 in C.fructicola lays the groundwork for understanding its role in pathogenicity.Based on reverse genetics principles,knockout and complement plasmids were constructed by using over-lap PCR method.The mutant strain ACfdot1 and the complement strain △Cfdot1/CfDOT1 were generated through genetic transformation,antibiotics screening and PCR amplification validation.Subsequent analysis revealed the nuclear localization of CfDot1.Biological phenotype determination indicates that the growth rate of the CfDOT1 gene knockout mutant does not exhibit significant difference in comparison with the wild-type and complement strains.However,there was a notable decrease in appressorium formation rate,as well as hypersensitivity to cell wall,H2O2,and endoplasmic reticulum stress agents,and therefore weakening pathogenicity.The histone methyltransferase CfDot1 plays a crucial role in regulation of spore production,appressorium formation,response to external stressors,and pathogenicity.关键词
油茶/果生刺盘孢/组蛋白赖氨酸甲基转移酶CfDot1/致病力Key words
Camellia oleifera/Colletotrichum fructicola/histone lysine methyltransferase CfDot1/pathogenicity引用本文复制引用
范晶晶,李冠辰,王子瑄,李河..组蛋白赖氨酸甲基转移酶CfDot1在果生刺盘孢中的功能[J].菌物学报,2025,44(2):55-67,13.基金项目
国家重点研发计划(2023YFD1401301)This work was supported by the National Key Research and Development Program of China(2023YFD1401301). （2023YFD1401301）
