菌物学报2025，Vol.44Issue(2)：94-112,19.DOI:10.13346/j.mycosystema.240221
药食兼用真菌松脂皱皮孔菌的生物学特性和抗氧化活性
Biological characteristics and antioxidant activities of both medicinal and edible Ischnoderma resinosum
摘要
Abstract
The polypore Ischnoderma resinosum is both medicinal and edible,having potential to be developed as health foods.The biological characteristics and antioxidant activities of a I.resinosum strain collected from Changbai Mts.,Jilin Province were studied.The ideal conditions for the mycelial growth of I.resinosum,including optimal carbon and nitrogen sources,inorganic salts,pH,and temperature,were systematically tested through single-factor and orthogonal experiments as well as Box-Behnken design under solid-state cultivation.Some indexes for its antioxidant activities were measured by spectrophotometry during liquid cultivation.Single-factor experiment results indicated that sucrose,malt extract,and KH2PO4 were the most favorable carbon source,nitrogen source,and inorganic salt,respectively,for the mycelial growth,particularly at pH 6.0 and temperature of 25 ℃.Further orthogonal test displayed that the impact extent on the mycelial growth rate were ranked as nitrogen source＞carbon source＞pH＞inorganic salt.The levels of the main factors that affect the mycelial growth rate were authenticated by the Box-Behnken design.I.resinosum could produce substantial quantities of polysaccharides,polyphenols,and ascorbic acids as well as possessed superoxide dismutase activities and the abilities to scavenge hydroxyl radicals,as observation under liquid culture.The optimal medium for mycelial growth were sucrose 20.0 g/L,malt extract 13.0 g/L,and KH2PO4 0.7 g/L.Under pH 5.0 and 25 ℃,the mycelial growth rate was(12.07±0.11)mm/d.I.resinosum antioxidant activity was confirmed by determination of several indexes in the liquid cultivation.关键词
药食兼用真菌/松脂皱皮孔菌/生物学特性/抗氧化活性Key words
medicinal and edible mushroom/Ischnoderma resinosum/biological characteristic/antioxidant activity引用本文复制引用
叶祎璠,金灿,唐禄鑫,王豪,季盈仪,刘璐,刘嘉诚,司静..药食兼用真菌松脂皱皮孔菌的生物学特性和抗氧化活性[J].菌物学报,2025,44(2):94-112,19.基金项目
北京市科技新星计划(20230484322) （20230484322）
国家自然科学基金(32270016,32070016,32470002) （32270016,32070016,32470002）
北京林业大学"国家级大学生创新创业训练计划"(202410022109,202310022109)This work was supported by the Beijing Nova Program(20230484322),the National Natural Science Foundation of China(32270016,32070016,32470002),and the National Undergraduate Training Programs for Innovation and Entrepreneurship(202410022109,202310022109). （202410022109,202310022109）
