菌物学报2025，Vol.44Issue(2)：113-124,12.DOI:10.13346/j.mycosystema.240208
灵芝子实体中粗多糖的制备及其抑制血管生成活性初步评价
Preparation of crude polysaccharides from Ganoderma lingzhi and preliminary evaluation of its angiogenic inhibitory activities
摘要
Abstract
Polysaccharide named as GL-P extracted from fruiting body of Ganoderma lingzhi GL0102 cultivated on bagasse was extracted by microwave-ultrasound and deproteinized by Sevag method and finally obtained through dialysis.The physicochemical characteristics and antioxidant activity of GL-P were analyzed,and its effect on angiogenesis of zebrafish peritoneal vein was evaluated.The results showed that the yield of GL-P was(22.51±1.29)g/kg.The polysaccharide content was(65.41±2.08)％,the protein content was(31.46±0.34)％,and the molecular weight was 1.27x104 g/mol.The monosaccharide composition was mainly xylose,arabinose,mannose,glucose and galactose,of which xylose and arabinose accounted for more than a half.The infrared spectrum analysis showed that GL-P was a polysaccharide-protein complex with β-glucan configuration.In vitro antioxidant activity evaluation showed that GL-P had antioxidant activity,and the antioxidant effect of 2 mg/mL GL-P was equivalent to that of 0.06-0.08 mg/mL VC.The safe concentration range of GL-P for zebrafish embryos should be less than 4.0 mg/mL,and GL-P can significantly inhibit the subintestinal veins of zebrafish embryos.This study provides reference for the development of G.lingzhi polysaccharide as a new high-efficiency and low-toxicity potential anti-tumor product.关键词
灵芝/多糖/单糖组成/抗氧化/血管生成Key words
Ganoderma lingzhi/polysaccharide/monosaccharide composition/antioxidation/angiogenesis引用本文复制引用
王庆福,黄清铧,王丽宁,胡彪,平兆华,孟飞..灵芝子实体中粗多糖的制备及其抑制血管生成活性初步评价[J].菌物学报,2025,44(2):113-124,12.基金项目
广东省科学院青年人才专项(2023GDASQNRC-0306) （2023GDASQNRC-0306）
广东省科学院发展专项资金项目(2024GDASZH-2024010102) （2024GDASZH-2024010102）
广州市科技计划(2024E04J0947) （2024E04J0947）
云浮市科技计划(2022010202)This work was supported by the Young Talent Project of GDAS(2023GDASQNRC-0306),the Project of Science and Technology Development of GDAS(2024GDASZH-2024010102),the Science and Technology Project of Guangzhou(2024E04J0947),and the Science and Technology Project ofYunfu(2022010202). （2022010202）
