菌物学报2025，Vol.44Issue(2)：125-137,13.DOI:10.13346/j.mycosystema.240216
响应面法优化广叶绣球菌蛋白制备工艺及其体外抗消化道肿瘤活性评价
Optimization for preparation process of the protein from Sparassis latifolia by response surface methodology and evaluation on its anti-gastrointestinal cancer activities in vitro
摘要
Abstract
The ethanol extract(SLEE),crude polysaccharide(SLCP)and total protein(SLTP)of Sparassis latifolia were prepared and their main constituent content was analyzed.The effects of SLEE,SLCP and SLTP on the growth of three different human gastrointestinal cancer(GI)cell lines,viz.HepG2,MGC-803 and HT-29 cells,were detected by using MTT assay.Response surface methodology was employed to optimize the preparation process of SLTP,and the inhibitory effects of different doses(12.5,25,50,100 and 200 μg/mL)of different protein successively precipitated by increasing saturation ammonium sulfate on the growth of HT-29 cells were determined.The results indicated that extraction rate of SLEE was(22.75±0.27)％,with total triterpene content of(11.40±0.20)％;SLCP extraction rate was(7.29±0.23)％,with total sugar content of(59.66±0.80)％;SLTP extraction rate was(8.80±0.57)％,with total protein content of(44.59±0.43)％.SLTP possessed the strongest inhibitory effect on the growth of the GI cancer cell lines,with the IC50 values(24 h)of 44.18,34.27 and 28.93 μg/mL against HepG2,MGC-803 and HT-29 cells,respectively.The optimal extraction process of SLTP was as follows:the concentration of sodium chloride was 0.15 mol/L,the ratio of solid to liquid was 1∶26,and the extraction time was 13 h.Under the optimized conditions,the average yield of SLTP was(11.03±0.07)％.Protein precipitated by 30％-50％saturation ammonium sulfate(SLSP-50)exhibited the most significant inhibitory activity on the growth of HT-29 cells,with the IC50 value(24 h)of 18.84 μg/mL.The protein of Sparassis latifolia possessed significant inhibitory property on the growth of GI cancer cells in vitro,being one of the main effective ingredients responsible for the anticancer effect.关键词
绣球菌/蛋白/响应面法/工艺优化/抗消化道肿瘤活性Key words
Sparassis latifolia/protein/response surface methodology/process optimization/anti-gastrointestinal cancer activity引用本文复制引用
李秀杰,冯彩玲,杨驰,林智,吴建国,吴岩斌..响应面法优化广叶绣球菌蛋白制备工艺及其体外抗消化道肿瘤活性评价[J].菌物学报,2025,44(2):125-137,13.基金项目
福建省自然科学基金(2022J01866) （2022J01866）
国家自然科学基金(81700524)This work was supported by the Natural Science Foundation of Fujian Province(2022J01866)and the National Natural Science Foundation of China(81700524). （81700524）
