检验医学与临床2025，Vol.22Issue(4)：445-450,456,7.DOI:10.3969/j.issn.1672-9455.2025.04.003
宫颈癌组织中CCCH型锌指蛋白13、着丝粒蛋白H的表达及临床意义
Expression of CCCH-type zinc finger protein 13 and centromere protein H in cervical cancer tissues and their clinical significance
摘要
Abstract
Objective To investigate the expression and clinical significance of CCCH-type zinc finger pro-tein 13(ZC3H13)and centromere protein H(CENPH)in cervical cancer tissues.Methods A total of 302 cervical cancer patients admitted to the hospital from February 2016 to February 2018 were included as reasearch subjects.Real-time quantitative PCR and immunohistochemical staining were used to detect the mR-NA and protein expression of ZC3H13 and CENPH in cervical cancer and adjacent tissues.The relationship between the expression levels of ZC3H13 mRNA and CENPH mRNA was analyzed.Additionally,the correla-tion between the expression of ZC3H13,CENPH and clinical pathological features was evaluated.Kaplan-Meier survival curves were used to compare the survival outcomes of patients with different expression levels of ZC3H13 and CENPH.Multivariate Cox regression analysis was performed to identify the prognostic factors affecting the survival of cervical cancer patients.Results Compared with adjacent tissues(1.18±0.25,1.03±0.20),the expression levels of ZC3H13 mRNA(3.34±0.46)and CENPH mRNA(3.03±0.51)in cancer tissues were significantly higher,with statistically significant differences(t=71.697,63.445,P<0.05).The positive rates of ZC3H13[70.20%(212/302)]and CENPH[66.87%(202/302)]were also high-er in cancer tissues compared with adjacent tissues[6.62%(20/302),5.96%(18/302),with statistically sig-nificant differences(χ2=257.993,246.403,P<0.05).The expression levels of ZC3H13 mRNA and CENPH mRNA in cervical cancer were positively correlated(r=0.775,P<0.001).The positive rates of ZC3H13 and CENPH in cancer tissues were significantly higher in patients with FIGO stage ⅡA,poorly differentiated tumors and lymph node metastasis compared with those with FIGO stage ⅠA-ⅠB,moderately to well-dif-ferentiated tumors and no lymph node metastasis,with statistically significant differences(P<0.05).There was a statistically significant difference in the survival curves between ZC3H13-positive and ZC3H13-negative patients(Log-rank χ2=9.785,P=0.002);similarly,there was a statistically significant difference in the sur-vival curves between CENPH-positive and CENPH-negative patients(Log-rank χ2=7.309,P=0.007).FI-GO stage,lymph node metastasis,degree of differentiation and the expression of ZC3H13 and CENPH in cer-vical cancer tissues were identified as prognostic factors for cervical cancer patients(P<0.05).Conclusion The ex-pression levels of ZC3H13 and CENPH elevate in cervical cancer,and both are involved in the disease progres-sion,serving as factors that influence the prognosis of cervical cancer.关键词
宫颈癌/CCCH型锌指蛋白13/着丝粒蛋白H/生存曲线/生存率Key words
cervical cancer/CCCH-type zinc finger protein 13/centromere protein H/survival curve/survival rate分类
医药卫生引用本文复制引用
程思,伍雯莹,胡静..宫颈癌组织中CCCH型锌指蛋白13、着丝粒蛋白H的表达及临床意义[J].检验医学与临床,2025,22(4):445-450,456,7.基金项目
湖北省卫生健康委员会第三批联合基金项目(WJ2019H480). （WJ2019H480）
