检验医学与临床2025，Vol.22Issue(4)：485-490,495,7.DOI:10.3969/j.issn.1672-9455.2025.04.011
T淋巴细胞亚群联合ALOX12B对高危型HPV感染病情自然转归的预测价值
The predictive value of T lymphocyte subsets combined with ALOX12B on the nature progression of high-risk HPV infection
摘要
Abstract
Objective To investigate the predictive value of T lymphocyte subsets combined with 12(R)-li-poxygenase(ALOX12B)in the nature progression of high-risk human papillomavirus(HPV)infection.Meth-ods A total of 179 patients with high-risk HPV infection admitted to the hospital from January 2020 to Feb-ruary 2021 were selected as research subjects.Based on the natural progression of their condition during a 2-year follow-up period,they were divided into the clearance group and the persistent positive group.Clinical da-ta,proportions of CD3+and CD4+T lymphocytes,the CD4+/CD8+T lymphocyte ratio(CD4+/CD8+)and ALOX12B mRNA levels were compared between the two groups.Logistic regression analysis was used to i-dentify factors influencing the natural progression of high-risk HPV infection.Receiver operating characteris-tic(ROC)curve was applied to evaluate the predictive value of T lymphocyte subsets and ALOX12B mRNA for the natural progression of high-risk HPV infection.Results During the 2-year follow-up,1 case was lost to follow-up among the 179 patients with high-risk HPV infection.Among the patients who were followed up,124 cases(69.66%)showed natural clearance,while 54 cases(30.34%)remained persistently positive(per-sistent positive group).Among the persistently positive patients,5 cases(9.26%)experienced disease pro-gression.The proportion of patients with biopsy results of cervical intraepithelial neoplasia(CIN)grade Ⅰ,and those with cytology≥atypical squamous cells of undetermined significance,as well as the high-risk HPV viral load in the persistent positive group were higher than those in the clearance group(P<0.05).The pro-portions of CD3+and CD4+T lymphocytes,as well as the CD4+/CD8+in the persistent positive group were lower than those in the clearance group(P<0.05),while the ALOX12B mRNA level was higher than that in the persistent positive group(P<0.05).Logistic regression analysis showed that,after adjusting for biopsy results,the proportions of CD3+and CD4+T lymphocytes,the CD4+/CD8+and ALOX12B mRNA remained factors influencing the natural progression of high-risk HPV infection(P<0.05).ROC curve analysis re-vealed that the combined prediction of CD3+T lymphocyte proportion,CD4+/CD8+and ALOX12B mRNA for the natural progression of high-risk HPV infection had an area under the curve of 0.928(95%CI:0.879-0.961).Conclusion The proportions of CD3+and CD4+T lymphocytes,the CD4+/CD8+and ALOX12B mR-NA are factors influencing the natural progression of high-risk HPV infection.The combination of T lympho-cyte subsets and ALOX12B mRNA can serve as a predictive approach for the natural progression of the dis-ease,providing valuable information for clinical decision-making.关键词
T淋巴细胞亚群/12(R)-脂加氧酶/高危型人乳头瘤病毒/持续感染/病情转归Key words
T lymphocyte subsets/12(R)-lipoxygenase/high-risk human papillomavirus/persistent infection/disease progression分类
医药卫生引用本文复制引用
刘敏,杨易,刘兰,夏春兰..T淋巴细胞亚群联合ALOX12B对高危型HPV感染病情自然转归的预测价值[J].检验医学与临床,2025,22(4):485-490,495,7.基金项目
四川省医学(青年创新)科研课题(S20601). （青年创新）
