可再生能源2025，Vol.43Issue(2)：143-151,9.
基于温和预处理玉米秸秆的酶解糖化工艺研究
Research on enzymatic hydrolysis and saccharification process of corn straw based on mild pretreatment
摘要
Abstract
Pretreatment and enzymatic hydrolysis of straw-type biomass are among the key technologies for its high-value conversion and utilization.Using corn stover as the raw material,pretreatment methods with formic acid,sodium chlorite,and alkaline hydrogen peroxide were studied and compared.Methods such as scanning electron microscopy,X-ray diffraction,and Fourier-transform infrared spectroscopy were employed to analyze the composition,morphology,crystal structure,and functional groups of corn straw before and after pretreatment.After pretreatment with formic acid,sodium chlorite,and alkaline hydrogen peroxide,the lignin removal rates were 66.71％,97.12％,and 91.88％respectively,and the enzymatic hydrolysis rates reached 63.26％,71.83％,and 95.14％respectively.Under the conditions where the cellulase dosage is 19.6 FPU/g,the xylanase dosage is 35.44 IU/g,and the Tween 80 dosage is 20.96 mg/g,the predicted enzymatic hydrolysis rate of corn straw pretreated with alkaline hydrogen peroxide is 95.57％,while the actual enzymatic hydrolysis rate is 94.42％.The optimal feeding strategy for high-solid enzymatic hydrolysis was an initial substrate concentration of 8％,with 4％of the substrate added at 6,12 h,and 24 h respectively.After 120 h of enzymatic hydrolysis,the enzymatic hydrolysis rate reached 87.57％.关键词
玉米秸秆/预处理/高固酶解/分批补料Key words
corn stover/pretreatment/high solid enzymatic hydrolysis/fed-batch分类
能源科技引用本文复制引用
张欢欢,林钰程,王圆圆,赵世强,杜朝军,赵子高,常春..基于温和预处理玉米秸秆的酶解糖化工艺研究[J].可再生能源,2025,43(2):143-151,9.基金项目
国家自然科学基金项目(22178328) （22178328）
南阳市协同创新重大专项(郑州大学南阳研究院)(21XTCX12002) （郑州大学南阳研究院）
河南省科技攻关项目(222102320059). （222102320059）
