南京师大学报（自然科学版）2025，Vol.48Issue(1)：66-74,9.DOI:10.3969/j.issn.1001-4616.2025.01.009
虾肝肠胞虫Septin的生物信息学分析及其在增殖中的潜在作用
Bioinformatics Analysis of Enterocytozoon hepatopenaei Septin and Its Potential Role in Proliferation
摘要
Abstract
Enterocytozoon hepatopenaei(EHP)is one of the significant pathogens in the shrimp aquaculture industry,but the molecular mechanisms of its division and proliferation remain unclear.This study aimed to elucidate the potential role of the EhpSeptin protein during EHP division by cloning and analyzing the gene sequence,expression,and localization of EhpSeptin during the division period of EHP.The results indicated that the EhpSeptin gene sequence encoded a protein with 265 amino acids,a molecular weight of approximately 30.4 kDa,and an isoelectric point of 4.75.An EhpSeptin prokaryotic expression vector was constructed,and high-purity expressed protein was obtained.Western blotting confirmed the consistency of the expressed protein with the native protein.Additionally,this study established chromogenic in situ hybridization(CISH)for the EhpSeptin gene to analyze its expression pattern in infected shrimp samples.The results demonstrated that the expression level of EhpSeptin increased with the intensity of EHP infection,suggesting its involvement in the EHP division process.This study is the first to report the cloning,expression,localization,and variations in the EhpSeptin protein during EHP infection,providing a theoretical foundation and technical means for exploring the molecular mechanisms of EHP division and preventing infection by this pathogen.关键词
虾肝肠胞虫/Septin/原核表达/显色原位杂交Key words
Enterocytozoon hepatopenaei/Septin/prokaryotic expression/chromogenic in situ hybridization分类
农业科技引用本文复制引用
张晟,乔毅,曹晓慧,张雷霆,成婕,吴海龙,沈辉..虾肝肠胞虫Septin的生物信息学分析及其在增殖中的潜在作用[J].南京师大学报（自然科学版）,2025,48(1):66-74,9.基金项目
江苏省"333"人才专项[(2022)]21、江苏海洋生物资源创新中心科研项目计划专项资金(2022YHTZZZ04)、江苏省渔业专项(2023-SJ-072). （2022）
