农业生物技术学报2025，Vol.33Issue(2)：271-284,14.DOI:10.3969/j.issn.1674-7968.2025.02.004
辣椒CNGC基因家族鉴定及表达分析
Identification and Expression Analysis of CNGC Gene Family in Pepper(Capsicum annuum)
摘要
Abstract
Plant cyclic nucleotide-gated ion channel(CNGC)plays an important role in plant growth,development and stress response.In order to investigate the function of CNGC gene family in pepper(Capsicum annuum),the CNGC family members of pepper were identified,and the physical and chemical properties,subcellular localization,phylogenetic development,structural characteristics,promoter cis-acting elements and gene expression patterns were analyzed.The results showed that a total of 14 CaCNGC gene family members were identified in pepper,which were not uniformly distributed on each chromosome.The isoelectric point of CaCNGC was 8.64～9.61,the molecular weight was 72.87～91.49 kD.The instability index was greater than 40,indicating that 14 members of CaCNGC were unstable proteins.All CaCNGC proteins were located in the plasma membrane,had transmembrane structures,and were hydrophilic proteins.The analysis of cis-element in promoter showed that,most of the CaCNGC contained cis-elements related to growth and development,hormone regulation,and abiotic stresses response.Transcriptome data analysis showed that most members of CaCNGC had low or no expression in leaves,flowers and fruits of pepper.Most members of CaCNGC were expressed under low temperature,high temperature,abscisic acid,gibberellin,hydrogen peroxide,indoleacetic acid,jasmonic acid and salt stress.qRT-PCR analysis showed that the expression of CaCNGC2,CaCNGC7 and CaCNGC10,was up-regulated under low and high temperature stress,which might be involved in the response of pepper to temperature stress.These results provide a theoretical reference for further investigating the function of CaCNGC gene family,and new insights into the molecular mechanisms of pepper tolerance to temperature stress.关键词
辣椒/环核苷酸门控离子通道(CNGC)/基因家族/生物信息学分析/表达分析Key words
Pepper/Cyclic nucleotide-gated ion channel(CNGC)/Gene family/Bioinformatic analysis/Expression analysis分类
农业科技引用本文复制引用
王田田,常雪瑞,王静..辣椒CNGC基因家族鉴定及表达分析[J].农业生物技术学报,2025,33(2):271-284,14.基金项目
山西省基础研究计划青年科学研究项目(202203021212451) （202203021212451）
山西省重点研发计划(202202140601006) （202202140601006）
山西省科技创新人才团队(202304051001018) （202304051001018）
山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目(SXBYKY2022015) （SXBYKY2022015）
山西农业大学博士科研启动项目(2021BQ115) （2021BQ115）
