农业生物技术学报2025，Vol.33Issue(2)：303-315,13.DOI:10.3969/j.issn.1674-7968.2025.02.006
葡萄响应灰霉菌侵染的差异microRNA分析及抗灰霉菌相关基因的筛选
Differential microRNA Analysis of Response to Botrytis cinerea Infection in Grapes(Vitis vinifera)and Screening of Genes Related to Botrytis cinerea Resistance
摘要
Abstract
MicroRNA(miRNA)mediated post transcriptional regulation plays a crucial role in plant responses to pathogens.In order to screen miRNAs in grapes(Vitis vinifera)that respond to Botrytis cinerea infection,this study infected grape leaves with B.cinerea spore suspension and constructed a small RNA library for high-throughput sequencing.A total of 171 known miRNAs and 251 new miRNAs were obtained in response to B.cinerea,of which 53 miRNAs showed differential expression after inoculation,especially miRNA399a,miRNA399b,and miRNA399h belonging to the miRNA399 family,which showed significant downregulation.The susceptibility of grapes to B.cinerea was determined by analyzing the phenotype,lesion area,and pathogenicity of leaves.8 key target genes were obtained by predicting the downstream target genes of the 3 key miRNAs mentioned above.qPCR analysis showed that the target genes of miRNA399b and miRNA399h were significantly upregulated when infected with B.cinerea.This study provides a theoretical basis for further understanding the resistance response of fruit trees to B.cinerea during domestication and for breeding resistant grape varieties.关键词
葡萄/miRNA/灰霉菌/高通量测序Key words
Grape/miRNA/Botrytis cinerea/High-throughput sequencing分类
农业科技引用本文复制引用
牛丽丽,杨清,宋洋波,何坤祖,周慧,武云龙,韩泽云,曹红燕,杜婷婷,刘丽媛,郗琳,孟冬..葡萄响应灰霉菌侵染的差异microRNA分析及抗灰霉菌相关基因的筛选[J].农业生物技术学报,2025,33(2):303-315,13.基金项目
自治区区域协同创新专项-上海合作组织科技伙伴计划及国际科技合作计划项目(2022E01066) （2022E01066）
国家资助博士后研究人员计划B档(GZB20230059) （GZB20230059）
