农业生物技术学报2025，Vol.33Issue(2)：427-442,16.DOI:10.3969/j.issn.1674-7968.2025.02.016
植酸酶微胶囊酶制剂的研发及其植物促生作用研究
Research of Phytase Microcapsule Enzyme Preparation and Its Plant Growth Promoting Effect
摘要
Abstract
Phosphorus in soil exists in complex forms,such as phytic acid,which is difficult to be absorbed and utilized by plants.This problem can be effectively solved through degrading soil phytic acid by phytase and releasing phosphorus.In this study,high-yielding phytase strains were selected from the soil,and the composition of the fermentation medium and culture conditions were optimized by the Response Surface Methodology.Phytase was prepared into microcapsule enzyme preparation with sodium alginate(SA)and CaCl2 as embedding and crosslinking agents,and its storage stability and growth-promoting effect on corn(Zea mays)were investigated.The results showed that a strain of phytase-producing bacteria was identified as Pseudomonas corrugata by 16S rRNA.The optimal medium composition and fermentation conditions were determined through fermentation optimization,and the phytase activity after optimization was(435.70±1.87)U/mL,which was increased by 82.16％compared with that before optimization.The microcapsule enzyme preparation prepared with 3％SA and 3％CaCl2 had the characteristics of good pellet formation and high enzyme activity.After 30 d of storage,the enzyme activity of(85.63±1.73)％remained.The experiment showed that microcapsule enzyme preparation significantly promoted corn growth,increasing corn seedling dry weight,plant height,root dry weight,root length and fresh seedling weight by 48.59％～304.46％.This study provided a method of strain screening,fermentation optimization and preparation of microcapsule enzyme preparation,which is of great significance for the extensive application of microcapsule enzyme preparations in agricultural production and new bacterial fertilizers.关键词
植酸酶/微胶囊酶制剂/发酵优化/响应面法/促生作用Key words
Phytase/Microcapsule enzyme preparation/Fermentation optimization/Response surface methodology/Growth-promoting分类
农业科技引用本文复制引用
张俐敏,刘天一,刘文佳,徐畅,莫继先..植酸酶微胶囊酶制剂的研发及其植物促生作用研究[J].农业生物技术学报,2025,33(2):427-442,16.基金项目
黑龙江省省属高等学校基本科研业务费(135409420) （135409420）
黑龙江省大学生创新创业训练计划(S202310232017) （S202310232017）
