四川农业大学学报2025，Vol.43Issue(1)：33-38,6.DOI:10.16036/j.issn.1000-2650.202408496
基于线粒体DNA ND6基因序列分析巨魾群体遗传多态性
Analysis of Bagarius yarrelli Genetic Polymorphism Based on Mitochondrial DNA ND6 Gene
摘要
Abstract
[Objective]This study aims to investigate the germplasm resources,genetic relationship,and genetic diversity of Bagarius yarrelli.[Method]The genetic diversity of B.yarrelli was studied by using mitochondrial ND6 gene of 60 individuals as a molecular marker in five populations of Bagariusyarrelli from the three rivers(Yuanjiang,Nujiang,and Lancangjiang)in Yunnan Province.[Result]The re-sults showed that the total length of mitochondrial ND6 was 525 bp,and 41 haplotypes were defined from 60 sequences.The average contents of A,T,C and G in all samples was 40.0%,13.7%,34.8%,and 11.5%,respectively.The A+T content was 53.7%and the percentage of C+G content was 46.3%.A total of 143 muta-tion sites,375 conserved sites,and 114 parsimony informative sites were detected.The nucleotide diversity and haplotype diversity were 0.087 69±0.002 35 and 0.960±0.017,respectively;and the average genetic distance between 41 haplotypes in 60 individuals was 0.101.[Conclusion]A phylogenetic tree was con-structed using the Neighbor-Joining(NJ)method for the ND6 gene sequence of 41 haplotypes of B.yarrelli and 7 other species of fish.It was found that B.yarrelli clustered into a single branch.关键词
巨魾/线粒体ND6/多态性/系统进化Key words
Bagarius yarrelli/mitochondrial ND6/polymorphism/phylogenetic evolution分类
农业科技引用本文复制引用
杜民,牛宝珍,施来凤,雷美榕,普文华,彭跃颖,张志仙,周甜,古金华..基于线粒体DNA ND6基因序列分析巨魾群体遗传多态性[J].四川农业大学学报,2025,43(1):33-38,6.基金项目
湖南文理学院博士启动项目(19BSQD39) （19BSQD39）
国家自然科学基金(31360638). （31360638）
