四川农业大学学报2025，Vol.43Issue(1)：39-47,9.DOI:10.16036/j.issn.1000-2650.202408492
急性低氧胁迫对大口黑鲈甘油三酯代谢的影响
Acute Hypoxic Stress:Effect on Triglyceride in Largemouth Bass(Micropterus salmoides)
摘要
Abstract
[Objective]This study aims to investigate the impact of acute hypoxia stress on triglyceride(TG)metabolism in largemouth bass.[Method]Three treatment groups[dissolved oxygen(DO):(7.0±0.15)mg/L,(3.5±0.12)mg/L,(1.2±0.2)mg/L]and six sampling time points(0,4,8,12,24 h and reoxygenation 12 h)were established in this experiment to measure biochemical markers related to TG metabolism,including TG,free fatty acid(FFA),lipase(LPS),and lipoprotein lipase(LPL)enzyme activity in liver,heart,brain,and mesenteric of largemouth bass following acute hypoxic stress.Additionally,the expression of genes re-lated to lipid metabolism,including peroxisome proliferator-activated receptor alpha(PPARα),peroxisome proliferator-activated receptor beta(PPARβ),peroxisome proliferator-activated receptor gamma(PPARγ),and lipoprotein lipase(LPL),was analyzed.[Result]After acute hypoxic stress,the contents of TG and FFA in four tissues of largemouth bass were significantly increased(P<0.05),and the activities of LPS enzymes in liver,heart and brain were significantly increased(P<0.05).The expression of PPARα gene was significantly up-regulated(P<0.05)in the four tissues at 8 h and 12 h of acute hypoxic stress.After reoxygenation,PPARβ gene expression in mesenteric fat returned to normal levels.PPARγ gene expression was significantly up-regulated in liver(P<0.05)and insignificant in the other three tissues.Hypoxia also induced differential expression of the LPL gene.[Conclusion]Under acute hypoxic stress,the oxidation of FFAs is suppressed,and the rates of TG synthesis and hydrolysis were altered in four different tissues of largemouth bass.TG hy-drolysis is active in liver,heart,and brain tissues,suggesting that largemouth bass may utilize glycerol through the 3-phosphoglycerate pathway to prioritize energy supply in these three tissues under anaerobic conditions.关键词
大口黑鲈/急性低氧胁迫/TG代谢/PPARs基因/LPL基因Key words
largemouth bass/acute hypoxia stress/TG metabolic/PPARs gene/LPL gene分类
农业科技引用本文复制引用
刘浩,赵浩惟,连文强,颜浩骁,刘巧,赵柳兰..急性低氧胁迫对大口黑鲈甘油三酯代谢的影响[J].四川农业大学学报,2025,43(1):39-47,9.基金项目
四川省自然科学基金(2024NSFSC2069). （2024NSFSC2069）
