四川农业大学学报2025，Vol.43Issue(1)：48-56,9.DOI:10.16036/j.issn.1000-2650.202410495
一株高致病性棘胸蛙源嗜水气单胞菌的全基因组测序及其感染后脾的超微病理损伤
Complete Genome Sequence of a Highly Pathogenic Aeromonas hydrophila Isolated from Quasipaa Spinose and Resultant Ultrastructural Pathology of Spleen
摘要
Abstract
[Objective]This study aims to explore the whole genome sequencing of a highly pathogenic Aeromonas hydrophila SZW-003 strain isolated from Quasipaa spinose,and to clarify the ultrastructural pathology after spleen infection,so as to provide a theoretical basis for etiological research and preven-tion and control of diseases caused by A.hydrophila.[Method]Genomic DNA was extracted from SZW-003 strain,the sequence was assembled,and the genome was annotated while observing the resultant dy-namic ultrastructural pathology of spleen injury.[Result]Strain SZW-003 possessed β-hemolysis char-acteristics.The results showed that SZW-003 contained a chromosome of 4 925 442 bp with an average G+C content of 60.64%and 4 473 coding genes,and 388 virulence factors were predicted in the VFDB database.The reticular cells in spleen of Quasipaa spinose were the first to be damaged with phagocytic pathogens inside after infection,and it was also the first cell type that swallowed pathogenic bacteria.Meanwhile,a large number of reticular cells were shed and transformed into macrophages and melanin macrophages.As the disease deteriorates,the reticular cells were swollen and lysed,the endothelial cells in the blood sinuses were necrotic in a large area,and a large number of scattered cell fragments and anomalous red blood cells could be seen in the splenic sinuses,and bacteria could be observed in the remaining macrophages[Conclusion]SZW-003 strain was a highly pathogenic Aeromonas hy-drophila.After invasion and infection,it caused splenic tissue structure disruption,with splenic reticular cells transforming into macrophages and melanin macrophages,causing severe damage.关键词
棘胸蛙/嗜水气单胞菌/全基因组/超微病理损伤Key words
Quasipaa spinose/Aeromonas hydrophila/whole genome/ultrastructural pathology分类
农业科技引用本文复制引用
任思宇,吴春艳,刘震坤,杨延辉,李新善..一株高致病性棘胸蛙源嗜水气单胞菌的全基因组测序及其感染后脾的超微病理损伤[J].四川农业大学学报,2025,43(1):48-56,9.基金项目
重庆市万州区科技局博士直通车项目(wzstc2022010) （wzstc2022010）
重庆市教委科研项目(KJQN202203514) （KJQN202203514）
重庆三峡职业学院创新团队(sxzy-2023td-006). （sxzy-2023td-006）
