四川农业大学学报2025，Vol.43Issue(1)：63-70,8.DOI:10.16036/j.issn.1000-2650.202405490
金鱼晶状缝碘泡虫病的组织病理观察和病原鉴定
Histopathological Observations and Pathogen Identification of A Disease Caused by Myxobolus lentisuturalis in Goldfish(Carassius auratus)
摘要
Abstract
[Objective]This study was conducted to investigate the etiology of a disease affecting goldfish at a farm in Sichuan,which caused muscle bulging and even skin breakdown severely compromised their ornamental value.[Method]The diagnosis was made based on histopathological examination,morpho-logical observation of parasites,and PCR identification.[Result]Gross pathological examination re-vealed that the primary symptom of the diseased fish was a hump-like lesion caused by the muscle bulge in front of the dorsal fin,which eventually ruptured and developed into an ulcer in the late stage of the disease.Histological analysis showed that the dorsal muscle tissue sustained the most severe damage,with numerous myoblasts in the lesion undergoing degeneration and necrosis,subsequently being re-placed by parasite spores.Morphological observation indicated that the parasite had an oval shape on the shell surface and a pear shape on the suture surface,measuring 10.3-13.3 μm×6.7-7.4 μm.It pos-sessed two equidistant polar vesicles measuring 3.4-4.2 μm×1.7-2.6 μm,with 4-5 rings of filaments perpendicularly entwined along the longitudinal axis of the vesicles,a morphology consistent with Myxobolus.Phylogenetic analysis of 18S rDNA amplification and sequencing confirmed that the parasite was Myxobolus lentisuturalis.[Conclusion]This study provides fundamental insights into the control of io-dine vesicular disease in goldfish.关键词
金鱼/晶状缝碘泡虫/组织病理/PCR检测Key words
goldfish/Myxobolus lentisuturalis/histopathology/PCR detection分类
农业科技引用本文复制引用
周佳文,杨磊,陈德芳,唐鹏,陈宗涛,李子翊..金鱼晶状缝碘泡虫病的组织病理观察和病原鉴定[J].四川农业大学学报,2025,43(1):63-70,8.基金项目
四川省农业产业技术体系创新团队项目(SCCXTD-2024-15). （SCCXTD-2024-15）
