四川农业大学学报2025，Vol.43Issue(1)：71-79,9.DOI:10.16036/j.issn.1000-2650.202411499
鲢鱼Cystatin C延缓4℃冷藏乌鱼片品质劣变及机制分析
Effect of Silver Carp Cystatin C on Delaying the Quality Deterioration of Mullet Fillets Stored at 4℃and Its Mechanism
摘要
Abstract
[Objective]Cystatin C of silver carp(HmCystatin C),which is widely distributed widely in tissues often discarded as offcuts in production,is a natural small protein with inhibitory effects on cysteine proteases and antibacterial properties.Given its potential as a biological preservative for aquatic products,this study aims to elucidate the underlying mechanism of HmCystatin C in delaying the quality deteriora-tion of refrigerated fillets stored at 4℃.[Method]The quality of mullet fillets treated with HmCystatin C was evaluated over a 5-day storage period at 4℃.The probable mechanisms of HmCystatin C in preser-vation,including its permeability into fish myocytes and its inhibitory effects on cathepsin B/L and domi-nant spoilage bacteria,were also explored.[Result]The results demonstrated that,compared to the control group(Con group),the treated group(Cys group)exhibited superior quality across various pa-rameters,including sensory scores,shear force,texture profile analysis(TPA),myofibril fragmentation index(MFI),structural protein integrity,and total bacterial counts.HmCystatin C was found to permeate into myocytes and significantly inhibit the activity of cathepsin B/L throughout the storage period.Addi-tionally,it exhibited notable antibacterial activity against dominant spoilage bacteria(DSB),including Aeromonas media and Staphylococcus saprophyticus,isolated from mullet fillets.[Conclusion]HmCystatin C significantly inhibited the activity of cathepsin B/L and the growth of DSB after permeating into myo-cytes,thereby effectively mitigating the quality deterioration of mullet fillets during storage at 4℃.关键词
HmCystatin C/冷藏乌鱼肉片品质/细胞内渗透性/酶活抑制/抑菌Key words
cystatin C of silver carp/quality of refrigerated mullet fillets/permeability into myocytes/inhibitory activity against Cathepsin B/L/antibacterial activity分类
农业科技引用本文复制引用
李龙飞,李树红,谭小千,王菲,李冉,蒋宇维,仝浩楠,黎成林,周子函..鲢鱼Cystatin C延缓4℃冷藏乌鱼片品质劣变及机制分析[J].四川农业大学学报,2025,43(1):71-79,9.基金项目
四川省自然科学基金(2025ZNSFSC0221) （2025ZNSFSC0221）
四川省自然科学基金(2023NSFSC1160). （2023NSFSC1160）
