肿瘤预防与治疗2025，Vol.38Issue(1)：1-10,10.DOI:10.3969/j.issn.1674-0904.2025.01.001
乳腺癌中POLR2J的表达情况及作用机制研究
Expression and Mechanism of POLR2J in Breast Cancer
摘要
Abstract
Objective:To analyze the expression and clinical significance of RNA polymerase Ⅱ subunit J(POLR2J)in breast cancer(BRCA),and investigate its biological roles in BRCA.Methods:POLR2J expression was analyzed at cellular and tissue levels in multiple databases.POLR2J expression in BRCA cell lines was analyzed using immunoblotting.The effect of POLR2J on prognosis and clinical features of BRCA was analyzed in multiple databases.We analyzed the effect of POLR2J on immune infiltration in BRCA using the cibersort function,and the mutational landscape of POLR2J in BRCA using the maftools package.Differentially expressed POLR2J genes were obtained using the limma package,POLR2J-related genes were identified using the cor.test function,and the intersecting genes were drawn and analyzed by protein-protein interaction networks and functional enrichment analysis.Then,71 BRCA patients who underwent surgical treatment in our hospital from January 2021 to December 2023 were selected,and the pathological tissue specimens of the patients were collected.The ex-pression levels of POLR2J in cancerous and paracancerous tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry staining,and their relationships to the clinicopathological parameters of the patients were detec-ted.Results:POLR2J was highly expressed in BRCA and was positively correlated to the poor prognosis of BRCA.The high expression of POLR2J predicted the negative expressions of estrogen receptor,progesterone receptor and human epidermal growth factor receptor 2 in BRCA.BRCA with high expression of POLR2J had a distinctly different immune microenvironment from that of BRCA with low expression of POLR2J.Pathway enrichment analysis showed that POLR2J was associated with ox-idative phosphorylation,mitochondrial gene expression,and electron transport chain in BRCA.BRCA with high POLR2J ex-pression had a higher TP53 mutation rate.High POLR2J expression was predictive of higher TNM stages and grades.POLR2J mRNA and protein levels were significantly higher in breast cancer tissue compared with paraneoplastic tissue.Con-clusion:POLR2J is a biomarker for predicting BRCA prognosis and influences oxidative phosphorylation,immune microenvi-ronment and gene mutations in BRCA.关键词
POLR2J/乳腺癌/免疫浸润/氧化磷酸化Key words
POLR2J/Breast cancer/Immune infiltration/Oxidative phosphorylation分类
生物科学引用本文复制引用
李理想,杨文灿,郝绪文,刘岩岩..乳腺癌中POLR2J的表达情况及作用机制研究[J].肿瘤预防与治疗,2025,38(1):1-10,10.基金项目
This study was supported by grants from Hefei Municipal Health Commission(No.Hwk2022yb023)and Third Hospital Affiliated to Anhui Medical University(No.2023sfy013). 合肥市卫生健康委2022年度应用医学研究项目(编号:Hwk2022yb023) （No.Hwk2022yb023）
安徽医科大学第三附属医院基础与临床合作研究提升计划培育项目(编号:2023sfy013) （编号:2023sfy013）
