山东农业科学2025，Vol.57Issue(1)：87-95,9.DOI:10.14083/j.issn.1001-4942.2025.01.012
硒与AM真菌对金线莲微量元素吸收及相关药用品质的影响
Effects of Selenium and Arbuscular Mycorrhizal Fungi on Trace Element Absorption and Medicinal Quality of Anoectochilus roxburghii
摘要
Abstract
In this study,the Anoectochilus roxburghii variety'Dayuanbao'was used as test material,the greenhouse simulation planting test method was used,and the combination treatments were set with three sele-nium(Se)levels(0,6,18 mg/L,referred to as Se0,Se1,Se2)and three fungi inoculation levels(no inoc-ulation,Rhizopus intraradices,Funneliformis mosseae,referred to as Am0,Am,,Am2).The effects of Se and arbuscular mycorrhizal(AM)fungi on the growth and development and contents of trace elements,main bio-active components and volatile substances of A.roxburghii were explored,hoping to provide a theoretical basis for the efficient cultivation and selenium-rich quality improvement of A.roxburghii.The results showed that ex-ogenous Se could increase the contents of chlorophyll,trace elements,main bioactive substances and volatile substances of A.roxburghii.Inoculation of AM fungi could further promote the growth and quality formation of A.roxburghii.On the whole,the extreme values of the above parameters occurred at Se,level.In the forms of soil selenium,under Se,level and the inoculation of AM fungi,the proportion of soluble selenium and ex-changeable selenium was relatively higher and the proportion of iron-manganese oxide-bound and organic-bound selenium was relatively lower.Combined with the relative growth rate,Se content and quality parameters of plant,SepAm,treatment performed better than Se1Am2.PCA analysis showed that Se content,bioactive components(total phenols,total flavonoids,anoectochilus glycosides,polysaccharides)contents and volatile substances(aldehydes,alcohols,ketones)contents were the main indexes to judge the effects of Se and AM fungi,and they were significantly positively correlated with soil available selenium content.In summary,the combined application of exogenous selenium and AM fungi could significantly promote the selenium activity in the rhizosphere soil of A.roxburghii,increase the effective selenium content,thereby increasing the selenium absorption,bioactive components accumulation and volatile substances contents of A.roxburghii,so as to im-prove its medicinal quality.The effect of 6 mg/L Se combined inoculation of R.intraradices was the best.关键词
金线莲/AM真菌/土壤硒形态/生物活性成分/挥发性物质Key words
Anoectochilus roxburghii/Arbuscular mycorrhizal fungi/Soil selenium form/Bioactive com-ponents/Volatile substances分类
农业科技引用本文复制引用
陈玮,甘志凯,谌希..硒与AM真菌对金线莲微量元素吸收及相关药用品质的影响[J].山东农业科学,2025,57(1):87-95,9.基金项目
国家自然科学基金项目(32260377,32001297) （32260377,32001297）
江西省教育厅重点资助项目(GJJ180638) （GJJ180638）
