山东农业科学2025，Vol.57Issue(1)：96-102,7.DOI:10.14083/j.issn.1001-4942.2025.01.013
1株半乳糖地质杆菌分离筛选及其对畜禽养殖固体有机废弃物的处理效果研究
Isolation of a Geobacillus galactosidasius Strain and Its Treatment Effects on Livestock and Poultry Solid Organic Wastes
摘要
Abstract
The study was aimed to obtain efficient degradation strains for solid organic wastes from live-stock and poultry breeding.A thermophilic strain BWTGW1.1 was isolated from pig manure sludge compost in Dongguan central slaughter house by dilution coating and high temperature culture methods.The strain was ini-tially identified as Geobacillus galactosidasius through morphological,physiological and biochemical analyses and 16S rRNA gene sequence construction analysis.The ability of salt resistance,high temperature resistance,high temperature protease production and treatment effect on livestock and poultry solid organic wastes of the BWTGW1.1 strain were further studied.The results showed that,in terms of salt tolerance,the BWTGW 1.1 strain grew well under the condition of 1.5％salt concentration,and the OD600 was 1.1 after cultured for 48 hours.In terms of high temperature resistance and high temperature protease production ability,the BWTGW1.1 strain could still survive after cultured for 12 hours at 85 ℃,and could produce hydrolyzed transparent circle after 48 hours at 60 ℃ with hydrolytic circle diameter/colony diameter greater than 2.0.In 116 m3 large verti-cal solid fermenter,BWTGW 1.1 bacterial solution+common bactericide could make the temperature of the pile reach 58 ℃ within 24 hours,which was 13 ℃ higher than that of common bactericide alone,and the high temperature of 60～70 ℃ could last 108 hours,which was only 60 hours in common bactericide treatment.The seed germination index of cucumber in BWTGW1.1 bacterial solution+common bactericide treatment was 86.75％after fermentation for 168 hours,which was 26.24％higher than that in common bactericide treatment.The results indicated that BWTGW 1.1 had potential application value in the compost fermentation process of pig manure sludge and other livestock and poultry solid organic wastes.关键词
半乳糖地质杆菌/嗜热高温菌/畜禽养殖固体废弃物/堆肥Key words
Geobacillus galactosidasius/Thermophilic bacteria/Livestock and poultry solid wastes/Compost分类
农业科技引用本文复制引用
谢小林,陈猛,李成江,周莲,刘玉敏,王勇,姚青,朱红惠..1株半乳糖地质杆菌分离筛选及其对畜禽养殖固体有机废弃物的处理效果研究[J].山东农业科学,2025,57(1):96-102,7.基金项目
韶关市科技计划项目(220805106270728) （220805106270728）
广州市科技计划项目(202201011832) （202201011832）
广东特支计划项目(2021JC06N628) （2021JC06N628）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2022SDZG09,2023SDZG09) （2022SDZG09,2023SDZG09）
