上海交通大学学报（医学版）2025，Vol.45Issue(1)：79-86,8.DOI:10.3969/j.issn.1674-8115.2025.01.009
重度牙周炎小鼠模型构建方法的优化与评价
Optimization and evaluation of mouse model construction method for severe periodontitis
摘要
Abstract
Objective·To investigate an optimal severe periodontitis mouse model by comparing two induction methods:simple ligature and ligature combined with injection of Porphyromonas gingivalis lipopolysaccharide(P.g.LPS).Methods·Fifteen C57BL/6 mice were divided into three groups:a healthy control group,a simple ligature-induced periodontitis group,and a ligature combined with P.g.LPS injection-induced periodontitis group.After 14 d,the following evaluations were conducted:tooth mobility and probing depth under a stereomicroscope;alveolar bone resorption[bone volume fraction,bone mineral density,the distance from the cemento-enamel junction(CEJ)to the alveolar bone crest(ABC),and the area between CEJ and ABC]analyzed via micro computed tomography(Micro-CT)and stereomicroscopic examination.The serum levels of inflammatory cytokines interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were assessed by enzyme-linked immunosorbent assay(ELISA).Results·Compared with the simple ligature group,mice in the ligature with P.g.LPS injection group exhibited significantly increased tooth mobility[(2.20±0.45)vs(1.40±0.55)]and probing depth[(1.05±0.21)mm vs(0.58±0.39)mm],with statistically significant differences(P＜0.05).The ligature with P.g.LPS injection group also demonstrated significantly reduced bone volume fraction[(16.44%±3.35%)vs(28.97%±7.90%)]and bone mineral density[(0.42±0.04)g/cm3 vs(0.55±0.08)g/cm3],as well as increased distance from CEJ to ABC[(0.88±0.03)mm vs(0.74±0.12)mm]and area between CEJ and ABC[(0.34±0.01)mm2 vs(0.30±0.02)mm2],all with statistically significant differences(all P＜0.05).Additionally,serum levels of TNF-α and IL-1β were significantly elevated in the ligature with P.g.LPS injection group compared to the simple ligature group(both P＜0.05).Conclusion·The method of ligature combined with continuous P.g.LPS injection is more effective for constructing a severe periodontitis mouse model,making it suitable for studying the progression and treatment of severe periodontitis.关键词
动物模型/牙周炎/小鼠/脂多糖Key words
animal model/periodontitis/mouse/lipopolysaccharide(LPS)分类
医药卫生引用本文复制引用
王佳璇,张骞骞,隋佰延,刘昕..重度牙周炎小鼠模型构建方法的优化与评价[J].上海交通大学学报（医学版）,2025,45(1):79-86,8.基金项目
国家自然科学基金(82271024) （82271024）
上海交通大学医学院附属第九人民医院交叉基金(JYJC202309) （JYJC202309）
上海市重中之重研究中心项目(2022ZZ01017) （2022ZZ01017）
中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-037). National Natural Science Foundation of China(82271024) （2019-I2M-5-037）
Cross Foundation of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine(JYJC202309) （JYJC202309）
Shanghai's Top Priority Research Center(2022ZZ01017) （2022ZZ01017）
Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2019-I2M-5-037). （2019-I2M-5-037）
