世界中西医结合杂志2025，Vol.20Issue(1)：126-135,10.DOI:10.13935/j.cnki.sjzx.250119
龙柴降血方对原发性血小板增多症转录组关键节点的调控
Longchai Jiangxue Formula Regulates Key Nodes of the Transcriptome in Treating Essential Thrombocythemia
摘要
Abstract
Objective To find the targets of Longchai Jiangxue Formula by building the essential thrombocythe-mia(ET)-related lncRNA-miRNA-mRNA regulatory network based on transcriptomics.Methods This study enrolled 28 patients with ET and 14 healthy individuals.Samples from 10 patients before treatment and 5 healthy individuals were used for high-throughput sequencing of lncRNAs,miRNAs,and mRNAs.Samples from the remaining 9 healthy individu-als and 18 patients with ET before treatment were used for qPCR to evaluate the results of high-throughput sequencing.Finally,the patients were assigned into an observation group(Longchai Jiangxue Formula+basic treatment)and a control group(basic treatment)based on a ratio of 1∶1.After 4 months of treatment,qPCR was employed to determine the expres-sion levels of key differentially expressed genes.Results A total of 5 lncRNAs,12 miRNAs,and 19 mRNAs were included in the lncRNA-miRNA-mRNA network,which included seven miRNA-lncRNA pairs and 35 miRNA-mRNA pairs.GO and KEGG enrichment analyses of differential mRNAs showed that platelet activation signaling pathway and PI3K-AKT signaling pathway were significantly enriched in ET.The key targets involved in the two pathways were F2R,ITGA2B,ITGB1,ITGB3,PTGS1,GP1BB,THBS1,and YWHAH,which were related to thrombosis and tumor proliferation.Then,qPCR was employed to verify the above targets.Compared with healthy individuals,the 18 patients with ET showed up-regulated expression levels of F2R,ITGA2B,ITGB1,ITGB3,GP1BB,THBS1,and YWHAH(P＜0.05),which were consistent with the high-throughput sequencing results,and there was no significant difference in the expression level of PTGS1(P＞0.05).Subsequently,qPCR was employed to determine the expression levels of the above key targets except PTGS1.Com-pared with the control group,the observation group showed down-regulated expression levels of THBS1,F2R,and ITGB3(P＜0.05).Conclusion Longchai Jiangxue Formula may regulate the platelet activation and PI3K-Akt signaling path-ways in the ET-related lncRNA-miRNA-mRNA network by down-regulating the expression levels of THBS1,F2R,and ITGB3.关键词
龙柴降血方/原发性血小板增多症/lncRNA/miRNA/mRNA/调控网络/转录组学Key words
Longchai Jiangxue Formula/Essential Thrombocythemia/lncRNA/miRNA/mRNA/Regulatory Net-work/Transcriptomics分类
医药卫生引用本文复制引用
赵霈,杨二鹏,孙妍,李雨蒙,郑茂凤,陈卓,王明镜,吕妍,胡晓梅..龙柴降血方对原发性血小板增多症转录组关键节点的调控[J].世界中西医结合杂志,2025,20(1):126-135,10.基金项目
国家自然科学基金面上项目(82174360) （82174360）
中国中医科学院科技创新工程重大攻关项目(CI2021A01702,CI2021A01708) （CI2021A01702,CI2021A01708）
