兽类学报2025，Vol.45Issue(1)：78-85,8.DOI:10.16829/j.slxb.150891
抗凝血剂对褐家鼠种群遗传多样性及抗药性的影响
The impact of anticoagulant rodenticides on the genetic diversity and an-ticoagulant resistance in the Norway rat population
摘要
Abstract
To understand the genetic diversity and the level of anticoagulant resistance in Norway rats(Rattus norvegi-cus)in Kashgar region,Xinjiang after continuous use of anticoagulant rodenticides,we collected Norway rat samples af-ter the application of the anticoagulant rodenticides in the year of 2019,2020,2022,and 2023.We first analyzed the dif-ferent mitochondrial Cyt b haplotypes and their frequencies in different temporal populations,as well as the phylogenetic relationship among different haplotypes.Then,we analyzed the Vkorc1 variations and their frequencies in the temporal populations and assessed the anticoagulant level of the populations.The results revealed a total of six different Cyt b hap-lotypes in 100 rats,and they clustered into two groups in the haplotype network.The highest and lowest haplotype diver-sity was found in the population of 2019 and 2023,respectively,indicating an obvious bottleneck effect.Some low-fre-quency haplotypes disappeared in the populations of 2020,2022,and 2023.Fst analysis suggested significant genetic differentiation between the population of 2022 and other temporal populations.Analysis of the Vkorc1 gene revealed one silent mutation Ile82Ile and no resistance-associated Vkorc1 missense mutations were found,indicating a low level of re-sistance in the local populations.All these findings suggest that the rat control in the entire village has led to a decrease in genetic diversity and significantly affected the genetic structure of the population.However,the rat populations in the studied area have not developed anticoagulant resistance yet,and the first-generation anticoagulant rodenticides are still effective.This study provides a reference for further research on the population structure of Norway rats and also pro-vides theoretical guidance for the use of anticoagulant rodenticides in local Norway rat populations.关键词
新疆维吾尔自治区/褐家鼠/抗凝血灭鼠剂/维生素K环氧化物还原酶复合体亚单位1基因/Cyt bKey words
Xinjiang Uygur Autonomous Region/Rattus norvegicus/Anticoagulant rodenticides/Vkorc1/Cyt b分类
生物科学引用本文复制引用
阿布都克尤木·卡德尔,孙婷,王大伟,刘晓辉,汪秀会,宋英..抗凝血剂对褐家鼠种群遗传多样性及抗药性的影响[J].兽类学报,2025,45(1):78-85,8.基金项目
农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室项目(KFJJ202303) （KFJJ202303）
新疆农业科学院自主培育专项(xjnkycxzx-2022003) （xjnkycxzx-2022003）
国家自然科学基金青年项目(32202333) （32202333）
"天池英才"引进计划 （）
